Pyflux

A desktop GUI for loading, filtering, visualizing, aligning, clustering, and exporting MINFLUX localization data from .npy files.

• Filter localizations by trace length, EFO, and CFR
• View data in interactive 2D/3D scatter plots
• Compare datasets in a multicolor viewer
• Generate heatmaps, Gaussian renderings, and TIFF exports
• Align datasets using MBM bead-based registration
• Run DBSCAN clustering on track-averaged localizations
• Export filtered data and summary statistics to CSV

Built with PySide6, Plotly, Pandas, SciPy, and Zarr.

Installation

• Download the current release
• Unzip
• For Macos: Allow app to run under Privacy & Security/Security -> Run anyway
• For Windows: Trust developer

Manual

Soon to come.

TODO

• add more filtering options
• fix mbm bead plots

Known bugs pyflux

• sometimes opens second window

License

MIT

Contact

SciLifeLab BioImage Informatics Unit

Developed by Maximilian Senftleben