Skip to content

Updates to public quilt data #166

New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Open
wants to merge 7 commits into
base: master
Choose a base branch
from
Open
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
15 changes: 11 additions & 4 deletions .gitignore
Original file line number Diff line number Diff line change
Expand Up @@ -115,7 +115,14 @@ venv.bak/
.mypy_cache/

# Results from assay-dev
./fish_morphology_code/processing/structure_organization/local_organization/train/data/
./fish_morphology_code/processing/structure_organization/local_organization/train/patches/
./fish_morphology_code/processing/structure_organization/output/
./fish_morphology_code/processing/structure_organization/results/
*.csv
fish_morphology_code/processing/structure_organization/local_organization/best_model
fish_morphology_code/processing/structure_organization/local_organization/train/data
fish_morphology_code/processing/structure_organization/local_organization/train/models
fish_morphology_code/processing/structure_organization/local_organization/train/patches
fish_morphology_code/processing/structure_organization/global_alignment/jinja2
fish_morphology_code/processing/structure_organization/tools/jinja2
fish_morphology_code/processing/structure_organization/notebooks/figs
fish_morphology_code/processing/structure_organization/output*
fish_morphology_code/processing/structure_organization/results*

5 changes: 3 additions & 2 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -39,7 +39,7 @@ contrast_and_segment quilt_data/metadata.csv quilt_data/supporting_files/channel

### Running cellprofiler to calculate single cell shape and texture features

Before running cellprofiler, download auto-contrasted images from quilt (ex. ```download_2D_contrasted --test=True```) into current working directory.
Before running cellprofiler, download auto-contrasted images from quilt (ex. ```download_2D_contrasted```) into current working directory.

Create an image set list in format accepted by cellprofiler's LoadData module.
```
Expand All @@ -48,7 +48,8 @@ make_cellprofiler_image_set \
--defaults_json fish_morphology_code/cellprofiler/cellprofiler_image_set_defaults.json \
--path_key rescaled_2D_fov_tiff_path \
--local_path ./quilt_data_contrasted_test \
Copy link
Collaborator

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

Should we remove test from the path since there is no longer a test set?

--out_loc ./test_image_set_list.csv
--out_loc ./test_image_set_list.csv \
--fish_type structure
```

Run cellprofiler pipeline in this repository using test image set list as input:
Expand Down
Loading