Skip to content

Commit

Permalink
updated paper url
Browse files Browse the repository at this point in the history
  • Loading branch information
danilexn committed Oct 30, 2024
1 parent e68bd11 commit 0b9bc75
Show file tree
Hide file tree
Showing 5 changed files with 5 additions and 5 deletions.
2 changes: 1 addition & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -12,7 +12,7 @@

# Open-ST: open-source spatial transcriptomics

### [🌐 website](https://rajewsky-lab.github.io/openst/latest) | [📜 paper](https://authors.elsevier.com/c/1jJckL7PXqR3U) | [🐁 datasets](https://rajewsky-lab.github.io/openst/latest/examples/datasets/)
### [🌐 website](https://rajewsky-lab.github.io/openst/latest) | [📜 paper](https://doi.org/10.1016/j.cell.2024.05.055) | [🐁 datasets](https://rajewsky-lab.github.io/openst/latest/examples/datasets/)

Open-ST is an open-source spatial transcriptomics method
with efficient whole-transcriptome capture at sub-cellular resolution (0.6 μm) at low cost
Expand Down
2 changes: 1 addition & 1 deletion docs/examples/getting_started.md
Original file line number Diff line number Diff line change
Expand Up @@ -6,7 +6,7 @@ leveraging the **Open-ST** workflow (experimental and computational).
## Datasets

We provide raw data, instructions on how to reproduce the preprocessing pipeline, the corresponding preprocessed
data (for comparison), and notebooks for some exploratory visualization and downstream analysis. We publicly provide mouse datasets, showcased in the [paper](https://authors.elsevier.com/c/1jJckL7PXqR3U).
data (for comparison), and notebooks for some exploratory visualization and downstream analysis. We publicly provide mouse datasets, showcased in the [paper](https://doi.org/10.1016/j.cell.2024.05.055).

<div class="grid cards" markdown>

Expand Down
2 changes: 1 addition & 1 deletion docs/experimental/capture_area_generation.md
Original file line number Diff line number Diff line change
Expand Up @@ -10,7 +10,7 @@ For instance, you can get ~360 capture areas sized 3x4 mm from a single **Illumi

When using an **Illumina® NovaSeq 6000 S4** flow cell (35 cycles), sequence the HDMI32-DraI library
(see in [Oligonucleotides](getting_started.md)) at a loading concentration of 200 pM.
Using 200 pM library, loaded according to the KAPA qPCR value, we obtained the following quality metrics for the barcoded fc_1 used in our [our paper](https://authors.elsevier.com/c/1jJckL7PXqR3U): Q30 >= 86%; PF = 78%; occupied = 97%. Although great results were achieved using this flow cell, 97% occupied is high. Consequently, we suggest to use a titration of library loading concentrations (one concentration per lane) when generating your first barcoded flow cell.
Using 200 pM library, loaded according to the KAPA qPCR value, we obtained the following quality metrics for the barcoded fc_1 used in our [our paper](https://doi.org/10.1016/j.cell.2024.05.055): Q30 >= 86%; PF = 78%; occupied = 97%. Although great results were achieved using this flow cell, 97% occupied is high. Consequently, we suggest to use a titration of library loading concentrations (one concentration per lane) when generating your first barcoded flow cell.

Sequence a single-end 37 cycle read, using Read1-DraI oligo as a custom primer.
Use a custom sequencing [recipe](../static/metadata_files/NovaSeq6000_S4_barcoding_seq_recipe.xml) that stops the run immediately after read 1 prior to on-instrument washes.
Expand Down
2 changes: 1 addition & 1 deletion docs/introduction.md
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
Welcome! Here, we provide comprehensive resources to help you generate and analyse Open-ST data in your lab.

Open-ST is a spatial transcriptomics method that enables efficient whole-transcriptome capture at subcellular resolution. In [our paper](https://authors.elsevier.com/c/1jJckL7PXqR3U), we have demonstrated Open-ST's wide applicability, showing robust transcriptome capture across various mouse and human tissues.
Open-ST is a spatial transcriptomics method that enables efficient whole-transcriptome capture at subcellular resolution. In [our paper](https://doi.org/10.1016/j.cell.2024.05.055), we have demonstrated Open-ST's wide applicability, showing robust transcriptome capture across various mouse and human tissues.
Our method is cost-efficient, straightforward to employ, and includes open-source software for seamless data processing and analysis.

Here, you can find detailed step-by-step descriptions of the experimental and the computational workflows. In a FAQ section we address commonly asked questions. Via our [discussion board] and our [chat], you can submit your own questions, and we will do our best to provide answers.
Expand Down
2 changes: 1 addition & 1 deletion docs/theme_override_home/home.html
Original file line number Diff line number Diff line change
Expand Up @@ -238,7 +238,7 @@ <h1><img src="static/logos/openst_logo_transparent_completeO.png" width="60px" s
<a href="{{ page.next_page.url | url }}" title="{{ page.next_page.title | striptags }}" class="md-button md-button--primary">
Get started
</a>
<a href="https://authors.elsevier.com/c/1jJckL7PXqR3U" title="Paper" class="md-button">
<a href="https://doi.org/10.1016/j.cell.2024.05.055" title="Paper" class="md-button">
Paper
</a><br>
<a href="{{ config.repo_url }}" title="{{ lang.t('source.link.title') }}" class="md-button">
Expand Down

0 comments on commit 0b9bc75

Please sign in to comment.