Contributor submission RebM

mite_data/data/108d2866-506d-11f1-9ef6-bb4e59819b6d.json

@@ -0,0 +1,73 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "comment": "RebM is the rebeccamycin sugar 4\u2032-O-methyltransferase that catalyzes the final tailoring step in rebeccamycin biosynthesis. It transfers a methyl group from S-adenosyl-L-methionine to the 4\u2032-hydroxy group of 4\u2032-demethylrebeccamycin, forming rebeccamycin and S-adenosyl-L-homocysteine.",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-05-15",
+            "contributors": [
+                "0009-0005-2489-2079"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "RebM",
+        "description": "SAM-dependent O-methyltransferase",
+        "references": [
+            "doi:10.1074/jbc.M800503200",
+            "doi:10.1002/cbic.200500504",
+            "doi:10.1271/bbb.67.127"
+        ],
+        "databaseIds": {
+            "uniprot": "Q8KZ94",
+            "genpept": "BAC10678.1",
+            "mibig": "BGC0000821"
+        },
+        "cofactors": {
+            "organic": [
+                "SAM"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Methylation"
+            ],
+            "description": "4\u2032-O-methylation of 4\u2032-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor.",
+            "reactionSMARTS": "[#8:1]=[#6:2]1-[#7:11]-[#6:9](=[#8:10])-[#6:8]2:[#6:3]-1:[#6:4]1:[#6:33]3:[#6:34]:[#6:35]:[#6:36]:[#6:37](-[Cl:38]):[#6:32]:3:[#7:20](\\[#6@:21]3-[#6@:30](/[#8:31])-[#6@@:28](\\[#8:29])-[#6@:26](/[#8:27])-[#6@@:23](\\[#6:24]-[#8:25])-[#8:22]-3):[#6:5]:1:[#6:6]1:[#7:19]:[#6:13]3:[#6:14](-[Cl:18]):[#6:15]:[#6:16]:[#6:17]:[#6:12]:3:[#6:7]:1:2>>[#8:1]=[#6:2]1-[#7:11]-[#6:9](=[#8:10])-[#6:8]2:[#6:3]-1:[#6:4]1:[#6:33]3:[#6:34]:[#6:35]:[#6:36]:[#6:37](-[Cl:38]):[#6:32]:3:[#7:20](\\[#6@:21]3-[#6@:30](/[#8:31])-[#6@@:28](\\[#8:29])-[#6@:26](/[#8:27]-[#6])-[#6@@:23](\\[#6:24]-[#8:25])-[#8:22]-3):[#6:5]:1:[#6:6]1:[#7:19]:[#6:13]3:[#6:14](-[Cl:18]):[#6:15]:[#6:16]:[#6:17]:[#6:12]:3:[#6:7]:1:2",
+            "reactions": [
+                {
+                    "substrate": "O=C1NC(=O)c2c1c1c3cccc(Cl)c3[nH]c1c1c2c2cccc(Cl)c2n1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O",
+                    "products": [
+                        "CO[C@H]1[C@H](O)[C@@H](O)[C@H](n2c3c(Cl)cccc3c3c4c(c5c6cccc(Cl)c6[nH]c5c32)C(=O)NC4=O)O[C@@H]1CO"
+                    ],
+                    "isIntermediate": false,
+                    "description": "4\u2032-O-methylation of 4\u2032-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor."
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "In vitro assay",
+                    "Knock-out studies",
+                    "Site-directed mutagenesis"
+                ],
+                "references": [
+                    "doi:10.1074/jbc.M800503200",
+                    "doi:10.1074/jbc.m800503200",
+                    "doi:10.1002/cbic.200500504"
+                ]
+            },
+            "databaseIds": {
+                "rhea": "27353",
+                "ec": "2.1.1.164"
+            }
+        }
+    ]
+}