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JoeVieira committed Sep 1, 2023
1 parent 02a93ad commit 39be56d
Showing 1 changed file with 16 additions and 16 deletions.
32 changes: 16 additions & 16 deletions src/main/scala/com/fulcrumgenomics/umi/GroupReadsByUmi.scala
Original file line number Diff line number Diff line change
Expand Up @@ -488,28 +488,28 @@ object Strategy extends FgBioEnum[Strategy] {
"""
)
class GroupReadsByUmi
(@arg(flag='i', doc="The input BAM file.") val input: PathToBam = Io.StdIn,
@arg(flag='o', doc="The output BAM file.") val output: PathToBam = Io.StdOut,
@arg(flag='f', doc="Optional output of tag family size counts.") val familySizeHistogram: Option[FilePath] = None,
@arg(flag='t', doc="The tag containing the raw UMI.") val rawTag: String = "RX",
@arg(flag='T', doc="The output tag for UMI grouping.") val assignTag: String = "MI",
@arg(flag='d', doc="Mark duplicates, duplicate bitflag will be set on non-representative reads.") val markDup: Boolean = false,
@arg(flag='D', doc="If -d is set, representative read assignment strategy, default = Sum Of Base Qualities.")
( @arg(flag='i', doc="The input BAM file.") val input: PathToBam = Io.StdIn,
@arg(flag='o', doc="The output BAM file.") val output: PathToBam = Io.StdOut,
@arg(flag='f', doc="Optional output of tag family size counts.") val familySizeHistogram: Option[FilePath] = None,
@arg(flag='t', doc="The tag containing the raw UMI.") val rawTag: String = "RX",
@arg(flag='T', doc="The output tag for UMI grouping.") val assignTag: String = "MI",
@arg(flag='d', doc="Mark duplicates, duplicate bitflag will be set on non-representative reads.") val markDup: Boolean = false,
@arg(flag='D', doc="If -d is set, representative read assignment strategy, default = Sum Of Base Qualities.")
val dupStrategy: DuplicateScoringStrategy.ScoringStrategy = DuplicateScoringStrategy.ScoringStrategy.SUM_OF_BASE_QUALITIES,
@arg(flag='S', doc="Include secondary reads.") val includeSecondary: Boolean = false,
@arg(flag='U', doc="Include supplementary reads.") val includeSupplementary: Boolean = false,
@arg(flag='m', doc="Minimum mapping quality for mapped reads.") val minMapQ: Int = 1,
@arg(flag='n', doc="Include non-PF reads.") val includeNonPfReads: Boolean = false,
@arg(flag='s', doc="The UMI assignment strategy.") val strategy: Strategy,
@arg(flag='e', doc="The allowable number of edits between UMIs.") val edits: Int = 1,
@arg(flag='l', doc= """The minimum UMI length. If not specified then all UMIs must have the same length,
@arg(flag='S', doc="Include secondary reads.") val includeSecondary: Boolean = false,
@arg(flag='U', doc="Include supplementary reads.") val includeSupplementary: Boolean = false,
@arg(flag='m', doc="Minimum mapping quality for mapped reads.") val minMapQ: Int = 1,
@arg(flag='n', doc="Include non-PF reads.") val includeNonPfReads: Boolean = false,
@arg(flag='s', doc="The UMI assignment strategy.") val strategy: Strategy,
@arg(flag='e', doc="The allowable number of edits between UMIs.") val edits: Int = 1,
@arg(flag='l', doc= """The minimum UMI length. If not specified then all UMIs must have the same length,
|otherwise discard reads with UMIs shorter than this length and allow for differing UMI lengths.
|""")
val minUmiLength: Option[Int] = None,
@arg(flag='x', doc= """
@arg(flag='x', doc= """
|DEPRECATED: this option will be removed in future versions and inter-contig reads will be
|automatically processed.""")
@deprecated val allowInterContig: Boolean = true
@deprecated val allowInterContig: Boolean = true
)extends FgBioTool with LazyLogging {
import GroupReadsByUmi._

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