update README

README.md

@@ -23,8 +23,18 @@ yak count -b37 -t16 -o pat.yak <(cat pat_1.fq.gz pat_2.fq.gz) <(cat pat_1.fq.gz
 yak count -b37 -t16 -o mat.yak <(cat mat_1.fq.gz mat_2.fq.gz) <(cat mat_1.fq.gz mat_2.fq.gz)
 hifiasm -o HG002.asm -t32 -1 pat.yak -2 mat.yak HG002-HiFi.fa.gz
 
-# Single-sample telomere-to-telomere assembly with HiFi, ultralong and Hi-C reads
+# Improve contiguity for diploid genome assembly by self-scaffolding
+hifiasm -o HG002.asm --dual-scaf --h1 read1.fq.gz --h2 read2.fq.gz HG002-HiFi.fq.gz
+
+# Preserve more telomeres for human genomes
+hifiasm -o HG002.asm --telo-m CCCTAA --h1 read1.fq.gz --h2 read2.fq.gz HG002-HiFi.fq.gz
+
+# Hybrid assembly with HiFi, ultralong and Hi-C reads
 hifiasm -o HG002.asm --h1 read1.fq.gz --h2 read2.fq.gz --ul ul.fq.gz HG002-HiFi.fq.gz
+
+# Single-sample telomere-to-telomere assembly for diploid human genomes
+hifiasm -o HG002.asm --dual-scaf --telo-m CCCTAA --h1 read1.fq.gz --h2 read2.fq.gz --ul ul.fq.gz HG002-HiFi.fq.gz
+
 ```
 See [tutorial][tutorial] for more details.