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R script to automatically select indexes combinations for single cell RNA sequencing, NovaSeqX

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Automatic indexes Selection for Nova SeqX

R script to automatically select indexes combinations for sequencing.

The criteria are

  • each index position pool, G<50%
  • each index position pool, C+T>30%.

The example used are randomly generated i7 and i5 instead of the real sets.

Assume we have 30 pairs and need to select 5 indexes to pool.

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R script to automatically select indexes combinations for single cell RNA sequencing, NovaSeqX

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