Question about %Fill in Peak Spot Table (Alignment) #698
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Pamou14160
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I am confused about the meaning of the %Fill column in the Peak Spot Table (Alignment) in MS-DIAL.
Initially, I assumed %Fill reflects:
• The peak count filter
• Or the N% detected parameter
(i.e., in how many samples a feature is present)
However, my observations do not match this interpretation.
For example:
• I set N% detected = 66% (feature must appear in at least 2 out of 3 samples per group)
• Yet I still observe features with %Fill ≈ 0.11 (very low presence rate)
• These features are not removed and do not appear to be tagged as low-quality.
Could someone clarify:
• What %Fill exactly represents?
• How it is calculated?
• How it differs from N% detected and peak count filtering?
• Whether gap filling affects this value?
Thank you for any clarification.
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