My samples have an 800x coverage depth, and Unicycler assembled some genomes with 75% to 93% completeness. Is there a specific parameter I can use to solve this? My command line was: unicycler -t $(nproc) --spades_options "--cov-cutoff auto" --min_fasta_length 200 -1 sample_1.fq.gz -2 sample_2.fq.gz -o sample_unicycler. SPAdes and Shovill are able to assemble them to near 100% completeness.
My samples have an 800x coverage depth, and Unicycler assembled some genomes with 75% to 93% completeness. Is there a specific parameter I can use to solve this? My command line was: unicycler -t $(nproc) --spades_options "--cov-cutoff auto" --min_fasta_length 200 -1 sample_1.fq.gz -2 sample_2.fq.gz -o sample_unicycler. SPAdes and Shovill are able to assemble them to near 100% completeness.