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CHANGELOG.md

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* Removed legacy future dependencies from Python2.7
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* Users can see the available system plugins, their commands and resource allocations: https://qiita.ucsd.edu/software/
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* Added qiime2.2020.06 to the system; which updated these plugins: qp-qiime2, qtp-biom, qtp-diversity, qtp-visualization
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* Shogun v1.0.8 for Metagenomic and Metatrascriptomics processing; this new version includes bowtie2 v2.4.1 as aligner and [Web of Life](https://biocore.github.io/wol/) and [rep200](ftp://ftp.ncbi.nlm.nih.gov/refseq/release/).
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Version 052020
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--------------

qiita_db/sql_connection.py

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@_checker
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def add(self, sql, sql_args=None, many=False):
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"""Add an sql query to the transaction
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"""Add a sql query to the transaction
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Parameters
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----------

qiita_pet/support_files/doc/source/dev/resource_allocation.rst

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simple.
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#. RESOURCE_PARAMS_COMMAND: This is the most common entry as it defines the allocation
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for an specific command name, like "Shogun v1.0.7" or "Beta diversity (phylogenetic)",
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for a specific command name, like "Shogun v1.0.7" or "Beta diversity (phylogenetic)",
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for the complete list of commands visit: `Qiita Software <https://qiita.ucsd.edu/software/>`__
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#. COMPLETE_JOBS_RESOURCE_PARAM: When a RESOURCE_PARAMS_COMMAND completes, it will define if the job
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finished successfully and a set of artifact(s) that need to be validated and then added to Qiita -

qiita_pet/support_files/doc/source/faq.rst

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names listed in the sample_name column in your preparation information file.
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What's a Qiita Artifact?
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------------------------
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A Qiita artifact is a collection of files and their summaries that represent the output
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or input of a processing or analytical command.
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For example a per_sample_FASTQ artifact will contain the per sample FASTQ files and their
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summary (if a user generated); while a BIOM artifact has the feature table as a biom file, a
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QIIME2 QZA, any other supporting files (like a phylogenetic tree for deblur or sortmerna_picked_otus.tgz
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for close reference picking), and summaries.
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How to convert Qiita files to QIIME2 artifacts?
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-----------------------------------------------
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How to cite Qiita?
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------------------
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If you use Qiita for processing, submition to EBI-ENA and/or its data for any published research, please include the following citation:
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If you use Qiita for processing, submission to EBI-ENA and/or its data for any published research, please include the following citation:
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**Qiita: rapid, web-enabled microbiome meta-analysis.**
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Antonio Gonzalez, Jose A. Navas-Molina, Tomasz Kosciolek, Daniel McDonald, Yoshiki Vázquez-Baeza, Gail Ackermann, Jeff DeReus, Stefan Janssen, Austin D. Swafford, Stephanie B. Orchanian, Jon G. Sanders, Joshua Shorenstein, Hannes Holste, Semar Petrus, Adam Robbins-Pianka, Colin J. Brislawn, Mingxun Wang, Jai Ram Rideout, Evan Bolyen, Matthew Dillon, J. Gregory Caporaso, Pieter C. Dorrestein & Rob Knight. Nature Methods, volume 15, pages 796–798 (2018);

qiita_pet/support_files/doc/source/processingdata/processing-recommendations.rst

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- Version: 0.99.8
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- Alignment file format: b6o (BLAST tabular output, i.e., `-outfmt 6`)
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- Website: https://github.com/knights-lab/BURST
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- Citation: Al-Ghalith, Gabriel and Dan Knights. BURST enables optimal exhaustive DNA alignment for big data. DOI 2017:doi.org/10.5281/zenodo.806850
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- Citation: Gabriel Al-Ghalith and Dan Knights. BURST enables optimal exhaustive DNA alignment for big data. DOI 2017:doi.org/10.5281/zenodo.806850
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- Note: Manuscript under review.
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#. UTree
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- Version: 2.0 RF
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- Alignment file format: custom mapping file
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- Website: https://github.com/knights-lab/UTree
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- Citation: Al-Ghalith, Gabriel and Dan Knights. Faster and lower-memory metagenomic profiling with UTree. DOI: 10.5281/zenodo.998252
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- Citation: Gabriel Al-Ghalith and Dan Knights. Faster and lower-memory metagenomic profiling with UTree. DOI: 10.5281/zenodo.998252
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Shogun reference databases
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^^^^^^^^^^^^^^^^^^^^^^^^^^
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- Strains: 89
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- Note: Nucleotide sequences per genome were concatenated with a linker of 20 "N"s.
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#. Rep200: NCBI representative and reference microbial genomes, corresponding to RefSeq release 200 (2020-05-14)
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- Genomes: 11,955
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- Nucleotides: 926,894
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- Basepairs: 62,823,581,921 (excluding gaps)
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- Numbers of taxonomic units:
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- Archaea: 419
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- Bacteria: 11080
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- Fungi: 320
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- Protozoa: 88
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- Viral: 48
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#. Rep94: NCBI representative and reference microbial genomes, corresponding to RefSeq release 94.
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- Domains: Bacteria, Archaea
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Sample processing guidelines for metatranscriptomic data
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^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
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Total community RNA extracted from samples contain both coding and non-coding RNA. Typically, ribosomal RNA make up >90% of the library if not depleted prior to library construction. Ribosomal depletion allows for mRNA enrichment. Even if you are dealing with ribosomal RNA subtracted cDNA libraries, there will be some
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Total community RNA extracted from samples contain both coding and non-coding RNA. Typically, ribosomal RNA make up
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>90% of the library if not depleted prior to library construction. Ribosomal depletion allows for mRNA enrichment. Even if
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you are dealing with ribosomal RNA subtracted cDNA libraries, there will be some
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residual ribosomal RNA in the libraries that you want to remove/separate from the non ribosomal RNA sequences.
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Ribosomal read filtering
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Latest SortMeRNA version: v2.1
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Input: Quality filtered Metatranscriptome reads (FASTA/FASTQ)
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Ribosomal reads are identified by searching against pre-curated rRNA databases. Currently, rRNA databases covering bacteria, archaea and eukarya were downloaded and indexed from `SILVA <https://www.arb-silva.de>`_ and `Rfam <https://rfam.xfam.org>`_.
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Ribosomal reads are identified by searching against pre-curated rRNA databases. Currently, rRNA databases covering bacteria, archaea and eukarya
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were downloaded and indexed from `SILVA <https://www.arb-silva.de>`_ and `Rfam <https://rfam.xfam.org>`_.
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Currently indexed databases and their clustering ids:
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SortMeRNA Usage
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^^^^^^^^^^^^^^^
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SortMeRNA filters the ribosomal from the non-ribosomal reads from the input sample dataset (via BLAST search)and outputs two fasta/q files containing the ribosomal and non-ribosomal reads respectively.
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SortMeRNA filters the ribosomal from the non-ribosomal reads from the input sample dataset (via BLAST search)and outputs two fasta/q files containing the
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ribosomal and non-ribosomal reads respectively.
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Additionally, a summary file showing the proportion of reads matching to each of the screened ribosomal databases can also be made available.
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Default options have been set to report only the best alignment per read reaching E-value.
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For non ribo-depleted samples (i.e. total RNA), the ribosomal reads obtained from SortMeRNA can be further used in taxonomic/compositional analysis.

qiita_ware/test/test_private_plugin.py

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'analysis': 3, 'merge_dup_sample_ids': True})
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# testing shape and get_resource_allocation_info as
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# build_analysis_files is an special case
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# build_analysis_files is a special case
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def _set_allocation(memory):
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with TRN:
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sql = """UPDATE qiita.processing_job_resource_allocation

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