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Copy file name to clipboardExpand all lines: CHANGELOG.md
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* Removed legacy future dependencies from Python2.7
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* Users can see the available system plugins, their commands and resource allocations: https://qiita.ucsd.edu/software/
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* Added qiime2.2020.06 to the system; which updated these plugins: qp-qiime2, qtp-biom, qtp-diversity, qtp-visualization
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* Shogun v1.0.8 for Metagenomic and Metatrascriptomics processing; this new version includes bowtie2 v2.4.1 as aligner and [Web of Life](https://biocore.github.io/wol/) and [rep200](ftp://ftp.ncbi.nlm.nih.gov/refseq/release/).
Antonio Gonzalez, Jose A. Navas-Molina, Tomasz Kosciolek, Daniel McDonald, Yoshiki Vázquez-Baeza, Gail Ackermann, Jeff DeReus, Stefan Janssen, Austin D. Swafford, Stephanie B. Orchanian, Jon G. Sanders, Joshua Shorenstein, Hannes Holste, Semar Petrus, Adam Robbins-Pianka, Colin J. Brislawn, Mingxun Wang, Jai Ram Rideout, Evan Bolyen, Matthew Dillon, J. Gregory Caporaso, Pieter C. Dorrestein & Rob Knight. Nature Methods, volume 15, pages 796–798 (2018);
Total community RNA extracted from samples contain both coding and non-coding RNA. Typically, ribosomal RNA make up >90% of the library if not depleted prior to library construction. Ribosomal depletion allows for mRNA enrichment. Even if you are dealing with ribosomal RNA subtracted cDNA libraries, there will be some
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Total community RNA extracted from samples contain both coding and non-coding RNA. Typically, ribosomal RNA make up
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>90% of the library if not depleted prior to library construction. Ribosomal depletion allows for mRNA enrichment. Even if
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you are dealing with ribosomal RNA subtracted cDNA libraries, there will be some
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residual ribosomal RNA in the libraries that you want to remove/separate from the non ribosomal RNA sequences.
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Ribosomal read filtering
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Ribosomal reads are identified by searching against pre-curated rRNA databases. Currently, rRNA databases covering bacteria, archaea and eukarya were downloaded and indexed from `SILVA <https://www.arb-silva.de>`_ and `Rfam <https://rfam.xfam.org>`_.
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Ribosomal reads are identified by searching against pre-curated rRNA databases. Currently, rRNA databases covering bacteria, archaea and eukarya
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were downloaded and indexed from `SILVA <https://www.arb-silva.de>`_ and `Rfam <https://rfam.xfam.org>`_.
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Currently indexed databases and their clustering ids:
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- silva-bacterial-16s-id 90%
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SortMeRNA Usage
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^^^^^^^^^^^^^^^
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SortMeRNA filters the ribosomal from the non-ribosomal reads from the input sample dataset (via BLAST search)and outputs two fasta/q files containing the ribosomal and non-ribosomal reads respectively.
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SortMeRNA filters the ribosomal from the non-ribosomal reads from the input sample dataset (via BLAST search)and outputs two fasta/q files containing the
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ribosomal and non-ribosomal reads respectively.
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Additionally, a summary file showing the proportion of reads matching to each of the screened ribosomal databases can also be made available.
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Default options have been set to report only the best alignment per read reaching E-value.
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For non ribo-depleted samples (i.e. total RNA), the ribosomal reads obtained from SortMeRNA can be further used in taxonomic/compositional analysis.
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