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DOC: Add polyG mention in the documentation (#3017)
* DOC: Add polyG mention in the documentation Fixes #2997 * DOC: Improve poly documentation
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qiita_pet/support_files/doc/source/processingdata/processing-recommendations.rst

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@@ -54,6 +54,12 @@ recommend that all sequence files go through adapter and quality control within
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subsequent analyses. Currently, the command removes adaptor sequences (only KAPA HyperPlus with iTru, which are compatible
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with Illumina TruSeq).
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Sequences generated with an instrument that relies on two-color chemistry (NextSeq, NovaSeq), need to undergo an additional
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quality control step. This step removes trailing G nucleotides which signify that the instrument has finished capturing new
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information. Per Illumina's specification, NovaSeq instruments have 3 quality levels (11, 25 and 37), and
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high-quality trailing Gs need to be removed. Typically this can be done in conjunction with adapter removal, with Atropos
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we recommend using the `--nextseq-trim 30` parameter.
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For host removal we currently support *Danio Rerio* (zebrafish), *Drosophila Melanogaster* (fruit fly), *Mus Musculus* (mouse),
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*Rattus Norvegicus* (rat), and Enterobacteria phage phiX174 (the Illumina spike-in control).
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