diff --git a/changelog/2574.bugfix.rst b/changelog/2574.bugfix.rst
new file mode 100644
index 00000000000..8c1923a634e
--- /dev/null
+++ b/changelog/2574.bugfix.rst
@@ -0,0 +1 @@
+Fix handling of ellipsis (...) in traceback comparisons for doctests
diff --git a/pyproject.toml b/pyproject.toml
index dce6a0870e1..292d8b950b7 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -417,7 +417,9 @@ markers = [
     "slow",
     # experimental mark for all tests using pexpect
     "uses_pexpect",
+    "doctest: mark tests related to doctests",
 ]
+doctest_optionflags = [ "ELLIPSIS" ]
 
 [tool.towncrier]
 package = "pytest"
diff --git a/src/_pytest/doctest.py b/src/_pytest/doctest.py
index 0dbef6056d7..9a4be9f04d3 100644
--- a/src/_pytest/doctest.py
+++ b/src/_pytest/doctest.py
@@ -649,6 +649,13 @@ def remove_prefixes(regex: re.Pattern[str], txt: str) -> str:
             if allow_number:
                 got = self._remove_unwanted_precision(want, got)
 
+            if "..." in want:
+                want_regex = re.escape(want).replace(r"\.\.\.", ".*")
+                print(f"Transformed WANT: {want_regex}")  # Debugging output
+                print(f"GOT: {got}")  # Debugging output
+                if re.fullmatch(want_regex, got, re.DOTALL):
+                    return True
+
             return super().check_output(want, got, optionflags)
 
         def _remove_unwanted_precision(self, want: str, got: str) -> str:
diff --git a/testing/test_doctest.py b/testing/test_doctest.py
index 1a852ebc82a..b8630063d76 100644
--- a/testing/test_doctest.py
+++ b/testing/test_doctest.py
@@ -1642,3 +1642,28 @@ def test_is_setup_py_different_encoding(tmp_path: Path, mod: str) -> None:
 def test_is_main_py(tmp_path: Path, name: str, expected: bool) -> None:
     dunder_main = tmp_path.joinpath(name)
     assert _is_main_py(dunder_main) == expected
+
+
+def test_doctest_wildcard(pytester):
+    """
+    Test that doctests with wildcards in tracebacks are handled correctly.
+    """
+    # Create a Python file with a function that raises a ValueError and includes a doctest.
+    pytester.makepyfile(
+        """
+        def _test_doctest_wildcard():
+            '''
+            >>> _test_doctest_wildcard()
+            Traceback (most recent call last):
+                ...
+            ValueError: An error occurred
+            '''
+            raise ValueError("An error occurred")
+        """
+    )
+
+    # Run pytest with doctest support enabled.
+    result = pytester.runpytest("--doctest-modules")
+
+    # Check that the test passed without failures.
+    result.assert_outcomes(passed=1, failed=0)