diff --git a/modules/nf-core/deepcell/mesmer/tests/main.nf.test b/modules/nf-core/deepcell/mesmer/tests/main.nf.test
index 248fa550..9d6e4904 100644
--- a/modules/nf-core/deepcell/mesmer/tests/main.nf.test
+++ b/modules/nf-core/deepcell/mesmer/tests/main.nf.test
@@ -17,7 +17,7 @@ nextflow_process {
                 """
                 input[0] = [
                     [ id: 'test_img' ],
-                    file(params.modules_testdata_base_path + '/modules/data/imaging/segmentation/cycif_tonsil_registered.ome.tif', checkIfExists: true)
+                    file(params.modules_testdata_base_path + '/modules/data/imaging/segmentation/cycif_tonsil_registered.ome.tif')
                 ]
                 input[1] = [
                     [:],