diff --git a/mite_data/data/108d2866-506d-11f1-9ef6-bb4e59819b6d.json b/mite_data/data/108d2866-506d-11f1-9ef6-bb4e59819b6d.json new file mode 100644 index 00000000..66f09701 --- /dev/null +++ b/mite_data/data/108d2866-506d-11f1-9ef6-bb4e59819b6d.json @@ -0,0 +1,73 @@ +{ + "accession": "MITE9999999", + "status": "pending", + "comment": "RebM is the rebeccamycin sugar 4\u2032-O-methyltransferase that catalyzes the final tailoring step in rebeccamycin biosynthesis. It transfers a methyl group from S-adenosyl-L-methionine to the 4\u2032-hydroxy group of 4\u2032-demethylrebeccamycin, forming rebeccamycin and S-adenosyl-L-homocysteine.", + "changelog": [ + { + "version": "1", + "date": "2026-05-15", + "contributors": [ + "0009-0005-2489-2079" + ], + "reviewers": [ + "BBBBBBBBBBBBBBBBBBBBBBBB" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "RebM", + "description": "SAM-dependent O-methyltransferase", + "references": [ + "doi:10.1074/jbc.M800503200", + "doi:10.1002/cbic.200500504", + "doi:10.1271/bbb.67.127" + ], + "databaseIds": { + "uniprot": "Q8KZ94", + "genpept": "BAC10678.1", + "mibig": "BGC0000821" + }, + "cofactors": { + "organic": [ + "SAM" + ] + } + }, + "reactions": [ + { + "tailoring": [ + "Methylation" + ], + "description": "4\u2032-O-methylation of 4\u2032-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor.", + "reactionSMARTS": "[#8:1]=[#6:2]1-[#7:11]-[#6:9](=[#8:10])-[#6:8]2:[#6:3]-1:[#6:4]1:[#6:33]3:[#6:34]:[#6:35]:[#6:36]:[#6:37](-[Cl:38]):[#6:32]:3:[#7:20](\\[#6@:21]3-[#6@:30](/[#8:31])-[#6@@:28](\\[#8:29])-[#6@:26](/[#8:27])-[#6@@:23](\\[#6:24]-[#8:25])-[#8:22]-3):[#6:5]:1:[#6:6]1:[#7:19]:[#6:13]3:[#6:14](-[Cl:18]):[#6:15]:[#6:16]:[#6:17]:[#6:12]:3:[#6:7]:1:2>>[#8:1]=[#6:2]1-[#7:11]-[#6:9](=[#8:10])-[#6:8]2:[#6:3]-1:[#6:4]1:[#6:33]3:[#6:34]:[#6:35]:[#6:36]:[#6:37](-[Cl:38]):[#6:32]:3:[#7:20](\\[#6@:21]3-[#6@:30](/[#8:31])-[#6@@:28](\\[#8:29])-[#6@:26](/[#8:27]-[#6])-[#6@@:23](\\[#6:24]-[#8:25])-[#8:22]-3):[#6:5]:1:[#6:6]1:[#7:19]:[#6:13]3:[#6:14](-[Cl:18]):[#6:15]:[#6:16]:[#6:17]:[#6:12]:3:[#6:7]:1:2", + "reactions": [ + { + "substrate": "O=C1NC(=O)c2c1c1c3cccc(Cl)c3[nH]c1c1c2c2cccc(Cl)c2n1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O", + "products": [ + "CO[C@H]1[C@H](O)[C@@H](O)[C@H](n2c3c(Cl)cccc3c3c4c(c5c6cccc(Cl)c6[nH]c5c32)C(=O)NC4=O)O[C@@H]1CO" + ], + "isIntermediate": false, + "description": "4\u2032-O-methylation of 4\u2032-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor." + } + ], + "evidence": { + "evidenceCode": [ + "Heterologous expression", + "In vitro assay", + "Knock-out studies", + "Site-directed mutagenesis" + ], + "references": [ + "doi:10.1074/jbc.M800503200", + "doi:10.1074/jbc.m800503200", + "doi:10.1002/cbic.200500504" + ] + }, + "databaseIds": { + "rhea": "27353", + "ec": "2.1.1.164" + } + } + ] +} \ No newline at end of file