diff --git a/mite_data/data/7932ffb0-5056-11f1-9ef6-bb4e59819b6d.json b/mite_data/data/7932ffb0-5056-11f1-9ef6-bb4e59819b6d.json new file mode 100644 index 00000000..6b3f2165 --- /dev/null +++ b/mite_data/data/7932ffb0-5056-11f1-9ef6-bb4e59819b6d.json @@ -0,0 +1,66 @@ +{ + "accession": "MITE9999999", + "status": "pending", + "comment": "RebG is the rebeccamycin indolocarbazole N-glycosyltransferase. It catalyzes N-glycosylation of the rebeccamycin aglycone, dichloroarcyriaflavin A, to form 4\u2032-demethylrebeccamycin ", + "changelog": [ + { + "version": "1", + "date": "2026-05-15", + "contributors": [ + "0009-0005-2489-2079" + ], + "reviewers": [ + "BBBBBBBBBBBBBBBBBBBBBBBB" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "RebG", + "description": "N-glycosyltransferase", + "references": [ + "doi:10.1002/cbic.200500504", + "doi:10.1271/bbb.67.127" + ], + "databaseIds": { + "uniprot": "Q8KHE4", + "genpept": "BAC10673.1", + "mibig": "BGC0000821" + } + }, + "reactions": [ + { + "tailoring": [ + "Glycosylation" + ], + "description": "N-glycosylation of dichloroarcyriaflavin A to 4\u2032-demethylrebeccamycin.", + "reactionSMARTS": "[#8:1]=[#6:2]1-[#6:7]2:[#6:8]3:[#6:17](:[#6:18]4:[#6:27](:[#6:6]:2-[#6:4](=[#8:5])-[#7:3]-1):[#6:26]1:[#6:20](:[#6:21](:[#6:23]:[#6:24]:[#6:25]:1)-[Cl:22]):[#7:19]:4):[#7:16]:[#6:15]1:[#6:9]:3:[#6:10]:[#6:11]:[#6:12]:[#6:13]:1-[Cl:14]>>[#8:1]=[#6:2]1-[#6:7]2:[#6:8]3:[#6:17](:[#6:18]4:[#6:27](:[#6:6]:2-[#6:4](=[#8:5])-[#7:3]-1):[#6:26]1:[#6:20](:[#6:21](:[#6:23]:[#6:24]:[#6:25]:1)-[Cl:22]):[#7:19]:4):[#7:16](/[#6@@]1-[#8]-[#6@](/[#6]-[#8])-[#6@@](\\[#8])-[#6@](/[#8])-[#6@]-1\\[#8]):[#6:15]1:[#6:9]:3:[#6:10]:[#6:11]:[#6:12]:[#6:13]:1-[Cl:14]", + "reactions": [ + { + "substrate": "O=C1NC(=O)c2c1c1c3cccc(Cl)c3[nH]c1c1[nH]c3c(Cl)cccc3c21", + "products": [ + "O=C1NC(=O)c2c1c1c3cccc(Cl)c3[nH]c1c1c2c2cccc(Cl)c2n1[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O" + ], + "isIntermediate": true, + "description": "N-glycosylation of dichloroarcyriaflavin A to 4\u2032-demethylrebeccamycin." + } + ], + "evidence": { + "evidenceCode": [ + "Heterologous expression", + "Inference from genomic data and chemical structure", + "Knock-out studies" + ], + "references": [ + "doi:10.1271/bbb.67.127", + "doi:10.1002/cbic.200500504", + "doi:10.1016/S1074-5521(02)00126-6" + ] + }, + "databaseIds": { + "rhea": "27397", + "ec": "4.3.3.5" + } + } + ] +} \ No newline at end of file