From 5763c968363c16297e8bf3fee23845a3d8985fd3 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Wed, 13 Mar 2024 18:53:24 -0400 Subject: [PATCH 1/7] add test for hmm poisson --- test/small_prediction_results_poisson.txt | 507 ++++++++++++++++++++++ test/test_HMMR_poisson.py | 77 ++++ 2 files changed, 584 insertions(+) create mode 100644 test/small_prediction_results_poisson.txt create mode 100644 test/test_HMMR_poisson.py diff --git a/test/small_prediction_results_poisson.txt b/test/small_prediction_results_poisson.txt new file mode 100644 index 00000000..ba1b48b0 --- /dev/null +++ b/test/small_prediction_results_poisson.txt @@ -0,0 +1,507 @@ +0.9999955641190514 4.435880897344368e-06 7.948854469221625e-18 +0.9999996825334214 3.174666504121522e-07 2.7778674328721742e-21 +0.9999996881056663 3.118943936180926e-07 1.9881569653975111e-22 +0.9999996881131696 3.118868542974087e-07 1.9532622029124232e-22 +0.9999996881131696 3.1188684410219215e-07 1.953215015499175e-22 +0.9999996881131696 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1.967253701436548e-22 +0.9999996880817921 3.1191816231965923e-07 2.0981767334639852e-22 +0.9999996649690381 3.3503093309821723e-07 1.0908103826393136e-20 +0.9999825824864104 1.7417513591871674e-05 2.8750313599971254e-16 diff --git a/test/test_HMMR_poisson.py b/test/test_HMMR_poisson.py new file mode 100644 index 00000000..58efdb76 --- /dev/null +++ b/test/test_HMMR_poisson.py @@ -0,0 +1,77 @@ + +import unittest +import pytest +# from MACS3.Signal.HMMR_HMM import * +import numpy as np +import numpy.testing as npt +import numpy as np +import hmmlearn +from hmmlearn.hmm import PoissonHMM +from sklearn import cluster +import json +# class hmmlearn.hmm.PoissonHMM(n_components=1, startprob_prior=1.0, transmat_prior=1.0, lambdas_prior=0.0, lambdas_weight=0.0,  +# algorithm='viterbi', random_state=None, n_iter=10, tol=0.01, verbose=False, params='stl', init_params='stl', implementation='log') + +# class hmmlearn.hmm.GaussianHMM(n_components=1, covariance_type='diag', min_covar=0.001, startprob_prior=1.0, transmat_prior=1.0,  +# means_prior=0, means_weight=0, covars_prior=0.01, covars_weight=1, algorithm='viterbi', random_state=None, n_iter=10, tol=0.01, verbose=False,  +# params='stmc', init_params='stmc', implementation='log') + +def hmm_training (training_data, training_data_lengths, n_states = 3, random_seed = 12345): + rs = np.random.RandomState(np.random.MT19937(np.random.SeedSequence(random_seed))) + hmm_model = PoissonHMM( n_components= n_states, random_state = rs, verbose = False ) + hmm_model = hmm_model.fit( training_data, training_data_lengths ) + assert hmm_model.n_features == 4 + return hmm_model + +def hmm_predict( signals, lens, hmm_model ): + predictions = hmm_model.predict_proba( signals, lens ) + return predictions + +class Test_HMM_train_poisson(unittest.TestCase): + def setUp( self ): + self.training_data = np.loadtxt("test/large_training_data.txt", delimiter="\t", dtype="float", usecols=(2,3,4,5)).astype(int).tolist() + self.training_data_lengths = np.loadtxt('test/large_training_lengths.txt', dtype="int").tolist() + self.expected_converged = True + self.not_expected_transmat = None + self.n_features = 4 + self.startprob = [0.83912489, 0.14996896, 0.01090615] + self.transmat = [[9.87606722e-01, 1.23932782e-02, 1.75299652e-11], + [1.76603580e-02, 9.64232293e-01, 1.81073490e-02], + [4.87992301e-14, 2.70319349e-02, 9.72968065e-01]] + self.lambdas = [[ 0.03809295, 0.62378578, 0.68739807, 0. ], + [ 0.23243362, 3.4420467, 4.256037, 0. ], + [ 2.58132377, 11.45924282, 8.13706237, 0. ]] + # for prediction + self.prediction_data = np.loadtxt("test/small_prediction_data.txt", delimiter="\t", dtype="float", usecols=(2,3,4,5)).astype(int).tolist() + self.prediction_data_lengths = np.loadtxt('test/small_prediction_lengths.txt', dtype="int").tolist() + self.predictions = np.loadtxt('test/small_prediction_results_poisson.txt', delimiter="\t", dtype="float").tolist() + + def test_training( self ): + # test hmm_training: + model = hmm_training(training_data = self.training_data, training_data_lengths = self.training_data_lengths, n_states = 3, random_seed = 12345) + print(model.startprob_) + print(model.transmat_) + print(model.lambdas_) + print(model.n_features) + self.assertEqual( model.monitor_.converged, self.expected_converged ) + self.assertNotEqual( model.transmat_.tolist(), self.not_expected_transmat ) + npt.assert_allclose( model.startprob_.tolist(), self.startprob ) + npt.assert_allclose(model.transmat_, self.transmat) + npt.assert_allclose(model.lambdas_, self.lambdas) + npt.assert_allclose(model.n_features, self.n_features) + + def test_predict( self ): + # test hmm_predict + hmm_model = PoissonHMM( n_components=3 ) + hmm_model.startprob_ = np.array(self.startprob) + hmm_model.transmat_ = np.array(self.transmat) + hmm_model.lambdas_ = np.array(self.lambdas) + hmm_model.n_features = self.n_features + predictions = hmm_predict( self.prediction_data, self.prediction_data_lengths, hmm_model ) + + # This is to write the prediction results into a file for 'correct' answer + # with open("test/small_prediction_results_poisson.txt","w") as f: + # for x,y,z in predictions: + # f.write( str(x)+"\t"+str(y)+"\t"+str(z)+"\n") + + npt.assert_allclose( predictions, self.predictions ) From a142a27ddd0b772e4ddb6029a201f0fd682c5fd8 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Thu, 14 Mar 2024 16:39:14 -0400 Subject: [PATCH 2/7] change hmm model to poisson, change ret_training_data to int type --- MACS3/Commands/hmmratac_cmd.py | 52 ++++++++++++++++++++----- MACS3/Signal/HMMR_Signal_Processing.pyx | 8 ++-- 2 files changed, 46 insertions(+), 14 deletions(-) diff --git a/MACS3/Commands/hmmratac_cmd.py b/MACS3/Commands/hmmratac_cmd.py index fe5de242..03008b3f 100644 --- a/MACS3/Commands/hmmratac_cmd.py +++ b/MACS3/Commands/hmmratac_cmd.py @@ -29,7 +29,8 @@ from MACS3.IO.Parser import BAMPEParser, BEDPEParser #BAMaccessor from MACS3.Signal.HMMR_EM import HMMR_EM from MACS3.Signal.HMMR_Signal_Processing import generate_weight_mapping, generate_digested_signals, extract_signals_from_regions -from MACS3.Signal.HMMR_HMM import hmm_training, hmm_predict, hmm_model_init, hmm_model_save +# from MACS3.Signal.HMMR_HMM import hmm_model_init, hmm_model_save +# from MACS3.Signal.HMMR_HMM import hmm_training, hmm_predict, hmm_model_init, hmm_model_save from MACS3.Signal.Region import Regions from MACS3.Signal.BedGraph import bedGraphTrackI @@ -42,7 +43,38 @@ # ------------------------------------ # Misc functions # ------------------------------------ - +import hmmlearn +from hmmlearn.hmm import PoissonHMM +def hmm_training (training_data, training_data_lengths, n_states = 3, random_seed = 12345): + rs = np.random.RandomState(np.random.MT19937(np.random.SeedSequence(random_seed))) + hmm_model = PoissonHMM( n_components= n_states, random_state = rs, verbose = False ) + hmm_model = hmm_model.fit( training_data, training_data_lengths ) + assert hmm_model.n_features == 4 + return hmm_model + +def hmm_predict( signals, lens, hmm_model ): + predictions = hmm_model.predict_proba( signals, lens ) + return predictions + +def hmm_model_save( model_file, hmm_model, hmm_binsize, i_open_region, i_nucleosomal_region, i_background_region ): + with open( model_file, "w" ) as f: + json.dump( {"startprob":hmm_model.startprob_.tolist(), + "transmat":hmm_model.transmat_.tolist(), + "lambdas":hmm_model.lambdas_.tolist(), + "n_features":int(hmm_model.n_features), + "i_open_region":int(i_open_region), + "i_background_region":int(i_background_region), + "i_nucleosomal_region":int(i_nucleosomal_region), + "hmm_binsize":int(hmm_binsize)}, f ) + +def hmm_model_init( model_file ): + with open( model_file ) as f: + m = json.load( f ) + hmm_model = PoissonHMM( n_components=3) + hmm_model.startprob_ = np.array(m["startprob"]) + hmm_model.transmat_ = np.array(m["transmat"]) + hmm_model.n_features = m["n_features"] + return [ hmm_model, m["i_open_region"], m["i_background_region"], m["i_nucleosomal_region"], m["hmm_binsize"] ] # ------------------------------------ # Main function # ------------------------------------ @@ -299,18 +331,18 @@ def run( args ): options.info( f"# Use Baum-Welch algorithm to train the HMM") - hmm_model = hmm_training( training_data, training_data_lengths, random_seed = options.hmm_randomSeed, covar="full" ) + hmm_model = hmm_training( training_data, training_data_lengths, random_seed = options.hmm_randomSeed, ) options.info( f"# HMM converged: {hmm_model.monitor_.converged}") # label hidden states - means_sum = np.sum( hmm_model.means_, axis=1 ) + lambdas_sum = np.sum( hmm_model.lambdas_, axis=1 ) # first, the state with the highest overall emission is the open state - i_open_region = np.where( means_sum == max(means_sum) )[0][0] + i_open_region = np.where( lambdas_sum == max(lambdas_sum) )[0][0] # second, the state with lowest overall emission is the bg state - i_background_region = np.where( means_sum == min(means_sum) )[0][0] + i_background_region = np.where( lambdas_sum == min(lambdas_sum) )[0][0] # last one is the nuc state (note it may not be accurate though i_nucleosomal_region = list(set([0, 1, 2]) - set([i_open_region, i_background_region]))[0] @@ -342,11 +374,11 @@ def run( args ): options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[0], hmm_model.transmat_[0]) ) options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[1], hmm_model.transmat_[1]) ) options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[2], hmm_model.transmat_[2]) ) - options.info( f"# HMM Emissions (mean): ") + options.info( f"# HMM Emissions (lambdas): ") options.info( "# {0[0]:>10s} {0[1]:>10s} {0[2]:>10s} {0[3]:>10s}".format( ["short", "mono", "di", "tri"] ) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[0], hmm_model.means_[0]) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[1], hmm_model.means_[1]) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[2], hmm_model.means_[2]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[0], hmm_model.lambdas_[0]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[1], hmm_model.lambdas_[1]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[2], hmm_model.lambdas_[2]) ) ############################################# diff --git a/MACS3/Signal/HMMR_Signal_Processing.pyx b/MACS3/Signal/HMMR_Signal_Processing.pyx index ba0a2f70..75b39a7f 100644 --- a/MACS3/Signal/HMMR_Signal_Processing.pyx +++ b/MACS3/Signal/HMMR_Signal_Processing.pyx @@ -171,10 +171,10 @@ cpdef list extract_signals_from_regions( list signals, object regions, int binsi for i in range( nn ): ret_training_bins.append( extracted_positions[0][i] ) ret_training_data.append( - [ max( 0.0001, extracted_data[0][i] ), - max( 0.0001, extracted_data[1][i] ), - max( 0.0001, extracted_data[2][i] ), - max( 0.0001, extracted_data[3][i] ) ] ) + [ int(max( 0.0001, extracted_data[0][i] )), + int(max( 0.0001, extracted_data[1][i] )), + int(max( 0.0001, extracted_data[2][i] )), + int(max( 0.0001, extracted_data[3][i] )) ] ) c = extracted_len[0][i] if counter != 0 and c != prev_c: ret_training_lengths.append( counter ) From de4fd742d351ee4770c60b342c52a0404e6b2c04 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Thu, 14 Mar 2024 16:56:53 -0400 Subject: [PATCH 3/7] try cmdlinetest with poisson output --- test/cmdlinetest | 17 +- ...500k_poisson_accessible_regions.gappedPeak | 729 ++++++++++++++++++ 2 files changed, 738 insertions(+), 8 deletions(-) create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_poisson_accessible_regions.gappedPeak diff --git a/test/cmdlinetest b/test/cmdlinetest index d1c457d5..b554bc3a 100755 --- a/test/cmdlinetest +++ b/test/cmdlinetest @@ -213,18 +213,19 @@ macs3 randsample -i $CHIPCONTIGS50K -n 100000 --seed 31415926 --outdir ${OUTPUTD echo "15. hmmratac" mkdir ${OUTPUTDIR_PREFIX}_run_hmmratac -macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_poisson --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log -macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log +# macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log -TRAINING_REGIONS_BED=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_training_regions.bed -HMM_MODEL=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_model.json +# TRAINING_REGIONS_BED=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_training_regions.bed +# HMM_MODEL=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_model.json -echo "15.1 hmmratac load training regions from bedfile" -macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log +# echo "15.1 hmmratac load training regions from bedfile" +# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log -echo "15.2 hmmratac load hmm model file" -macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log +# echo "15.2 hmmratac load hmm model file" +# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log echo "16. search for errors or warnings in log files" flag=0 diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_accessible_regions.gappedPeak new file mode 100644 index 00000000..38810eb7 --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_accessible_regions.gappedPeak @@ -0,0 +1,729 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 45180 45720 peak_2 0 . 45290 45590 0 1 300 110 0 0 0 +chrI 60720 61940 peak_3 0 . 61130 61290 0 1 160 410 0 0 0 +chrI 67960 69790 peak_4 0 . 68170 68960 0 1 790 210 0 0 0 +chrI 70930 71590 peak_5 0 . 71090 71510 0 1 420 160 0 0 0 +chrI 139030 139300 peak_6 0 . 139070 139210 0 1 140 40 0 0 0 +chrI 140570 142240 peak_7 0 . 141550 141950 0 1 400 980 0 0 0 +chrI 165050 165900 peak_8 0 . 165490 165830 0 1 340 440 0 0 0 +chrI 166090 166480 peak_9 0 . 166200 166370 0 1 170 110 0 0 0 +chrI 180850 181500 peak_10 0 . 181040 181310 0 1 270 190 0 0 0 +chrI 182330 182740 peak_11 0 . 182450 182670 0 1 220 120 0 0 0 +chrI 189850 192060 peak_12 0 . 190020 190670 0 1 650 170 0 0 0 +chrII 5290 6510 peak_13 0 . 5520 6370 0 1 850 230 0 0 0 +chrII 36120 36870 peak_14 0 . 36410 36620 0 1 210 290 0 0 0 +chrII 44380 45530 peak_15 0 . 45050 45500 0 1 450 670 0 0 0 +chrII 60780 61140 peak_16 0 . 60860 61100 0 1 240 80 0 0 0 +chrII 89420 90130 peak_17 0 . 89550 89880 0 1 330 130 0 0 0 +chrII 135690 136450 peak_18 0 . 135750 136290 0 1 540 60 0 0 0 +chrII 140440 141060 peak_19 0 . 140600 140840 0 1 240 160 0 0 0 +chrII 164190 164430 peak_20 0 . 164220 164400 0 1 180 30 0 0 0 +chrII 164790 165460 peak_21 0 . 165030 165390 0 1 360 240 0 0 0 +chrII 167890 168430 peak_22 0 . 167950 168300 0 1 350 60 0 0 0 +chrII 193180 194060 peak_23 0 . 193650 193870 0 1 220 470 0 0 0 +chrII 201980 203470 peak_24 0 . 202380 203340 0 2 170,200 400,1160 0 0 0 +chrII 221290 221920 peak_25 0 . 221420 221720 0 1 300 130 0 0 0 +chrII 227090 227400 peak_26 0 . 227170 227310 0 1 140 80 0 0 0 +chrII 251050 251650 peak_27 0 . 251330 251520 0 1 190 280 0 0 0 +chrII 332460 332760 peak_28 0 . 332500 332700 0 1 200 40 0 0 0 +chrII 336800 337660 peak_29 0 . 336930 337160 0 1 230 130 0 0 0 +chrII 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371260 371640 peak_291 0 . 371290 371530 0 1 240 30 0 0 0 +chrVII 384070 385300 peak_292 0 . 384560 385030 0 1 470 490 0 0 0 +chrVII 397790 398490 peak_293 0 . 397870 398390 0 1 520 80 0 0 0 +chrVII 401350 401710 peak_294 0 . 401490 401660 0 1 170 140 0 0 0 +chrVII 405310 405940 peak_295 0 . 405510 405650 0 1 140 200 0 0 0 +chrVII 412910 413650 peak_296 0 . 413260 413490 0 1 230 350 0 0 0 +chrVII 423010 423500 peak_297 0 . 423100 423320 0 1 220 90 0 0 0 +chrVII 428120 428400 peak_298 0 . 428140 428380 0 1 240 20 0 0 0 +chrVII 438310 439140 peak_299 0 . 438500 438770 0 1 270 190 0 0 0 +chrVII 483000 484290 peak_300 0 . 483330 483880 0 1 550 330 0 0 0 +chrVII 528150 528980 peak_301 0 . 528670 528780 0 1 110 520 0 0 0 +chrVII 536030 536830 peak_302 0 . 536120 536480 0 1 360 90 0 0 0 +chrVII 546470 547840 peak_303 0 . 547310 547680 0 1 370 840 0 0 0 +chrVII 555450 555830 peak_304 0 . 555490 555650 0 1 160 40 0 0 0 +chrVII 576400 576950 peak_305 0 . 576620 576800 0 1 180 220 0 0 0 +chrVII 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190 0 0 0 +chrXVI 424890 425940 peak_697 0 . 425200 425760 0 1 560 310 0 0 0 +chrXVI 431420 432420 peak_698 0 . 431700 432280 0 1 580 280 0 0 0 +chrXVI 450730 451450 peak_699 0 . 450790 451150 0 1 360 60 0 0 0 +chrXVI 453280 454000 peak_700 0 . 453510 453830 0 1 320 230 0 0 0 +chrXVI 454310 455370 peak_701 0 . 454960 455220 0 1 260 650 0 0 0 +chrXVI 463750 464220 peak_702 0 . 464010 464150 0 1 140 260 0 0 0 +chrXVI 481860 482430 peak_703 0 . 482100 482350 0 1 250 240 0 0 0 +chrXVI 502370 503250 peak_704 0 . 502480 503080 0 2 150,350 110,360 0 0 0 +chrXVI 519980 520610 peak_705 0 . 520260 520510 0 1 250 280 0 0 0 +chrXVI 526720 527480 peak_706 0 . 526980 527180 0 1 200 260 0 0 0 +chrXVI 572270 572560 peak_707 0 . 572330 572450 0 1 120 60 0 0 0 +chrXVI 585860 586590 peak_708 0 . 586100 586260 0 1 160 240 0 0 0 +chrXVI 641180 641910 peak_709 0 . 641250 641790 0 1 540 70 0 0 0 +chrXVI 644340 645800 peak_710 0 . 645050 645690 0 1 640 710 0 0 0 +chrXVI 678330 679440 peak_711 0 . 678610 679300 0 1 690 280 0 0 0 +chrXVI 694640 695540 peak_712 0 . 694980 695280 0 1 300 340 0 0 0 +chrXVI 700050 700370 peak_713 0 . 700080 700320 0 1 240 30 0 0 0 +chrXVI 731850 732250 peak_714 0 . 731890 732210 0 1 320 40 0 0 0 +chrXVI 769130 769450 peak_715 0 . 769180 769370 0 1 190 50 0 0 0 +chrXVI 770520 771080 peak_716 0 . 770880 771060 0 1 180 360 0 0 0 +chrXVI 794270 795060 peak_717 0 . 794620 794880 0 1 260 350 0 0 0 +chrXVI 809540 810340 peak_718 0 . 809830 810210 0 1 380 290 0 0 0 +chrXVI 821970 822540 peak_719 0 . 822090 822380 0 1 290 120 0 0 0 +chrXVI 828650 829580 peak_720 0 . 828830 829520 0 1 690 180 0 0 0 +chrXVI 840140 841060 peak_721 0 . 840280 840830 0 2 310,120 140,570 0 0 0 +chrXVI 844730 845540 peak_722 0 . 844900 845190 0 1 290 170 0 0 0 +chrXVI 855400 856300 peak_723 0 . 855770 856190 0 1 420 370 0 0 0 +chrXVI 856780 857080 peak_724 0 . 856820 857000 0 1 180 40 0 0 0 +chrXVI 860240 860900 peak_725 0 . 860250 860740 0 1 490 10 0 0 0 +chrXVI 880230 880530 peak_726 0 . 880270 880430 0 1 160 40 0 0 0 +chrXVI 927510 928500 peak_727 0 . 927870 928180 0 1 310 360 0 0 0 +chrXVI 942450 942850 peak_728 0 . 942570 942730 0 1 160 120 0 0 0 From 02f39c1a0a2cfb01efe43572c969a1bcf6e7e327 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Thu, 14 Mar 2024 17:04:50 -0400 Subject: [PATCH 4/7] try removing other hmmratac standard results --- ...ac_yeast500k_accessible_regions.gappedPeak | 929 ------------------ ...st500k_bedpe_accessible_regions.gappedPeak | 929 ------------------ ...ad_hmm_model_accessible_regions.gappedPeak | 929 ------------------ ...ning_regions_accessible_regions.gappedPeak | 929 ------------------ 4 files changed, 3716 deletions(-) delete mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak delete mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak delete mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_load_hmm_model_accessible_regions.gappedPeak delete mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_load_training_regions_accessible_regions.gappedPeak diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak deleted file mode 100644 index a877edea..00000000 --- a/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak +++ /dev/null @@ -1,929 +0,0 @@ -track name="peak" description="peak" type=gappedPeak nextItemButton=on -chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 -chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 -chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 0 -chrI 60100 62170 peak_4 0 . 60900 61820 0 2 570,270 800,1450 0 0 0 -chrI 62620 63520 peak_5 0 . 62630 62760 0 1 130 10 0 0 0 -chrI 67960 69560 peak_6 0 . 68170 69180 0 1 1010 210 0 0 0 -chrI 70980 71590 peak_7 0 . 71080 71510 0 1 430 100 0 0 0 -chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 -chrI 139050 139300 peak_9 0 . 139070 139270 0 1 200 20 0 0 0 -chrI 140550 142040 peak_10 0 . 141060 141970 0 2 210,430 510,990 0 0 0 -chrI 165240 165900 peak_11 0 . 165400 165840 0 1 440 160 0 0 0 -chrI 166010 166450 peak_12 0 . 166190 166390 0 1 200 180 0 0 0 -chrI 180720 181640 peak_13 0 . 181040 181310 0 1 270 320 0 0 0 -chrI 182150 182740 peak_14 0 . 182420 182670 0 1 250 270 0 0 0 -chrI 189840 192080 peak_15 0 . 190020 191780 0 1 1760 180 0 0 0 -chrI 210910 211270 peak_16 0 . 211060 211190 0 1 130 150 0 0 0 -chrI 220210 220830 peak_17 0 . 220320 220750 0 1 430 110 0 0 0 -chrII 0 930 peak_18 0 . 10 140 0 1 130 10 0 0 0 -chrII 5470 6500 peak_19 0 . 5510 6450 0 1 940 40 0 0 0 -chrII 36140 36670 peak_20 0 . 36380 36640 0 1 260 240 0 0 0 -chrII 44200 45530 peak_21 0 . 44990 45510 0 1 520 790 0 0 0 -chrII 60840 61110 peak_22 0 . 60860 61100 0 1 240 20 0 0 0 -chrII 87810 88050 peak_23 0 . 87910 88020 0 1 110 100 0 0 0 -chrII 89380 90090 peak_24 0 . 89540 89880 0 1 340 160 0 0 0 -chrII 116870 117410 peak_25 0 . 116940 117240 0 1 300 70 0 0 0 -chrII 135710 136450 peak_26 0 . 135740 136290 0 1 550 30 0 0 0 -chrII 140490 141210 peak_27 0 . 140600 141180 0 2 420,120 110,570 0 0 0 -chrII 159820 160770 peak_28 0 . 159840 160430 0 1 590 20 0 0 0 -chrII 164190 164420 peak_29 0 . 164200 164410 0 1 210 10 0 0 0 -chrII 164930 165660 peak_30 0 . 164990 165510 0 1 520 60 0 0 0 -chrII 167480 168410 peak_31 0 . 167910 168340 0 1 430 430 0 0 0 -chrII 193160 194220 peak_32 0 . 193620 193900 0 1 280 460 0 0 0 -chrII 202010 203360 peak_33 0 . 202200 203350 0 2 740,240 190,1100 0 0 0 -chrII 216430 217270 peak_34 0 . 216500 217040 0 1 540 70 0 0 0 -chrII 221310 221830 peak_35 0 . 221420 221700 0 1 280 110 0 0 0 -chrII 227090 227390 peak_36 0 . 227100 227350 0 1 250 10 0 0 0 -chrII 251070 251620 peak_37 0 . 251210 251540 0 1 330 140 0 0 0 -chrII 259930 260500 peak_38 0 . 260030 260240 0 1 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586100 586260 0 1 160 250 0 0 0 -chrXVI 641190 642000 peak_900 0 . 641210 641780 0 1 570 20 0 0 0 -chrXVI 643520 643700 peak_901 0 . 643530 643690 0 1 160 10 0 0 0 -chrXVI 644300 645750 peak_902 0 . 645040 645700 0 1 660 740 0 0 0 -chrXVI 653630 654070 peak_903 0 . 653830 654050 0 1 220 200 0 0 0 -chrXVI 678370 679380 peak_904 0 . 678480 679320 0 1 840 110 0 0 0 -chrXVI 694640 695550 peak_905 0 . 694980 695440 0 1 460 340 0 0 0 -chrXVI 699540 700360 peak_906 0 . 700070 700330 0 1 260 530 0 0 0 -chrXVI 719360 719750 peak_907 0 . 719410 719520 0 1 110 50 0 0 0 -chrXVI 731850 732230 peak_908 0 . 731890 732220 0 1 330 40 0 0 0 -chrXVI 769160 769390 peak_909 0 . 769170 769380 0 1 210 10 0 0 0 -chrXVI 770650 771070 peak_910 0 . 770830 771060 0 1 230 180 0 0 0 -chrXVI 794180 794980 peak_911 0 . 794620 794890 0 1 270 440 0 0 0 -chrXVI 809560 810320 peak_912 0 . 809740 810220 0 1 480 180 0 0 0 -chrXVI 812800 813050 peak_913 0 . 812820 812990 0 1 170 20 0 0 0 -chrXVI 822000 822520 peak_914 0 . 822090 822380 0 1 290 90 0 0 0 -chrXVI 828520 829600 peak_915 0 . 828830 829540 0 1 710 310 0 0 0 -chrXVI 834320 834850 peak_916 0 . 834370 834500 0 1 130 50 0 0 0 -chrXVI 840140 840890 peak_917 0 . 840280 840830 0 2 320,170 140,520 0 0 0 -chrXVI 844600 845320 peak_918 0 . 844900 845230 0 1 330 300 0 0 0 -chrXVI 854940 856320 peak_919 0 . 855770 856190 0 1 420 830 0 0 0 -chrXVI 856800 857210 peak_920 0 . 856820 857170 0 2 190,110 20,260 0 0 0 -chrXVI 860240 860900 peak_921 0 . 860250 860780 0 1 530 10 0 0 0 -chrXVI 880230 880520 peak_922 0 . 880260 880430 0 1 170 30 0 0 0 -chrXVI 899510 900620 peak_923 0 . 899820 900280 0 1 460 310 0 0 0 -chrXVI 900650 901460 peak_924 0 . 900820 901020 0 1 200 170 0 0 0 -chrXVI 921030 921430 peak_925 0 . 921220 921370 0 1 150 190 0 0 0 -chrXVI 927420 928440 peak_926 0 . 927870 928180 0 1 310 450 0 0 0 -chrXVI 942500 942840 peak_927 0 . 942550 942750 0 1 200 50 0 0 0 -chrXVI 947790 948010 peak_928 0 . 947870 948000 0 1 130 80 0 0 0 diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak deleted file mode 100644 index a877edea..00000000 --- a/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak +++ /dev/null @@ -1,929 +0,0 @@ -track name="peak" description="peak" type=gappedPeak nextItemButton=on -chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 -chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 -chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 0 -chrI 60100 62170 peak_4 0 . 60900 61820 0 2 570,270 800,1450 0 0 0 -chrI 62620 63520 peak_5 0 . 62630 62760 0 1 130 10 0 0 0 -chrI 67960 69560 peak_6 0 . 68170 69180 0 1 1010 210 0 0 0 -chrI 70980 71590 peak_7 0 . 71080 71510 0 1 430 100 0 0 0 -chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 -chrI 139050 139300 peak_9 0 . 139070 139270 0 1 200 20 0 0 0 -chrI 140550 142040 peak_10 0 . 141060 141970 0 2 210,430 510,990 0 0 0 -chrI 165240 165900 peak_11 0 . 165400 165840 0 1 440 160 0 0 0 -chrI 166010 166450 peak_12 0 . 166190 166390 0 1 200 180 0 0 0 -chrI 180720 181640 peak_13 0 . 181040 181310 0 1 270 320 0 0 0 -chrI 182150 182740 peak_14 0 . 182420 182670 0 1 250 270 0 0 0 -chrI 189840 192080 peak_15 0 . 190020 191780 0 1 1760 180 0 0 0 -chrI 210910 211270 peak_16 0 . 211060 211190 0 1 130 150 0 0 0 -chrI 220210 220830 peak_17 0 . 220320 220750 0 1 430 110 0 0 0 -chrII 0 930 peak_18 0 . 10 140 0 1 130 10 0 0 0 -chrII 5470 6500 peak_19 0 . 5510 6450 0 1 940 40 0 0 0 -chrII 36140 36670 peak_20 0 . 36380 36640 0 1 260 240 0 0 0 -chrII 44200 45530 peak_21 0 . 44990 45510 0 1 520 790 0 0 0 -chrII 60840 61110 peak_22 0 . 60860 61100 0 1 240 20 0 0 0 -chrII 87810 88050 peak_23 0 . 87910 88020 0 1 110 100 0 0 0 -chrII 89380 90090 peak_24 0 . 89540 89880 0 1 340 160 0 0 0 -chrII 116870 117410 peak_25 0 . 116940 117240 0 1 300 70 0 0 0 -chrII 135710 136450 peak_26 0 . 135740 136290 0 1 550 30 0 0 0 -chrII 140490 141210 peak_27 0 . 140600 141180 0 2 420,120 110,570 0 0 0 -chrII 159820 160770 peak_28 0 . 159840 160430 0 1 590 20 0 0 0 -chrII 164190 164420 peak_29 0 . 164200 164410 0 1 210 10 0 0 0 -chrII 164930 165660 peak_30 0 . 164990 165510 0 1 520 60 0 0 0 -chrII 167480 168410 peak_31 0 . 167910 168340 0 1 430 430 0 0 0 -chrII 193160 194220 peak_32 0 . 193620 193900 0 1 280 460 0 0 0 -chrII 202010 203360 peak_33 0 . 202200 203350 0 2 740,240 190,1100 0 0 0 -chrII 216430 217270 peak_34 0 . 216500 217040 0 1 540 70 0 0 0 -chrII 221310 221830 peak_35 0 . 221420 221700 0 1 280 110 0 0 0 -chrII 227090 227390 peak_36 0 . 227100 227350 0 1 250 10 0 0 0 -chrII 251070 251620 peak_37 0 . 251210 251540 0 1 330 140 0 0 0 -chrII 259930 260500 peak_38 0 . 260030 260240 0 1 210 100 0 0 0 -chrII 336830 337840 peak_39 0 . 336930 337190 0 1 260 100 0 0 0 -chrII 341560 342430 peak_40 0 . 341610 342350 0 2 370,250 50,540 0 0 0 -chrII 350440 351640 peak_41 0 . 350860 351010 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556190 peak_57 0 . 555600 556080 0 1 480 160 0 0 0 -chrII 561060 561200 peak_58 0 . 561080 561190 0 1 110 20 0 0 0 -chrII 564840 565580 peak_59 0 . 564960 565170 0 1 210 120 0 0 0 -chrII 586270 586430 peak_60 0 . 586280 586410 0 1 130 10 0 0 0 -chrII 588910 589600 peak_61 0 . 589250 589510 0 1 260 340 0 0 0 -chrII 592880 593120 peak_62 0 . 592890 593110 0 1 220 10 0 0 0 -chrII 595630 595920 peak_63 0 . 595650 595890 0 1 240 20 0 0 0 -chrII 604090 604440 peak_64 0 . 604220 604370 0 1 150 130 0 0 0 -chrII 605860 606290 peak_65 0 . 605890 606140 0 1 250 30 0 0 0 -chrII 614020 616030 peak_66 0 . 614150 615470 0 2 340,500 130,950 0 0 0 -chrII 618320 618960 peak_67 0 . 618650 618770 0 1 120 330 0 0 0 -chrII 624930 625630 peak_68 0 . 625010 625270 0 1 260 80 0 0 0 -chrII 628510 628840 peak_69 0 . 628530 628770 0 1 240 20 0 0 0 -chrII 642570 643190 peak_70 0 . 642820 643150 0 1 330 250 0 0 0 -chrII 644890 645450 peak_71 0 . 644930 645220 0 1 290 40 0 0 0 -chrII 647070 647450 peak_72 0 . 647300 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a/test/standard_results_hmmratac/hmmratac_yeast500k_load_hmm_model_accessible_regions.gappedPeak +++ /dev/null @@ -1,929 +0,0 @@ -track name="peak" description="peak" type=gappedPeak nextItemButton=on -chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 -chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 -chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 0 -chrI 60100 62170 peak_4 0 . 60900 61820 0 2 570,270 800,1450 0 0 0 -chrI 62620 63520 peak_5 0 . 62630 62760 0 1 130 10 0 0 0 -chrI 67960 69560 peak_6 0 . 68170 69180 0 1 1010 210 0 0 0 -chrI 70980 71590 peak_7 0 . 71080 71510 0 1 430 100 0 0 0 -chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 -chrI 139050 139300 peak_9 0 . 139070 139270 0 1 200 20 0 0 0 -chrI 140550 142040 peak_10 0 . 141060 141970 0 2 210,430 510,990 0 0 0 -chrI 165240 165900 peak_11 0 . 165400 165840 0 1 440 160 0 0 0 -chrI 166010 166450 peak_12 0 . 166190 166390 0 1 200 180 0 0 0 -chrI 180720 181640 peak_13 0 . 181040 181310 0 1 270 320 0 0 0 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225690 0 1 250 10 0 0 0 -chrV 241960 242700 peak_273 0 . 242150 242540 0 1 390 190 0 0 0 -chrV 259920 260350 peak_274 0 . 260150 260260 0 1 110 230 0 0 0 -chrV 268390 269130 peak_275 0 . 268420 268570 0 1 150 30 0 0 0 -chrV 270260 270600 peak_276 0 . 270280 270520 0 1 240 20 0 0 0 -chrV 284440 285090 peak_277 0 . 284510 285050 0 1 540 70 0 0 0 -chrV 303430 303840 peak_278 0 . 303600 303750 0 1 150 170 0 0 0 -chrV 305910 306270 peak_279 0 . 305940 306260 0 1 320 30 0 0 0 -chrV 311180 312180 peak_280 0 . 311510 312160 0 1 650 330 0 0 0 -chrV 334590 336790 peak_281 0 . 335420 335980 0 1 560 830 0 0 0 -chrV 342470 343100 peak_282 0 . 342480 342800 0 1 320 10 0 0 0 -chrV 349130 350180 peak_283 0 . 349450 349850 0 1 400 320 0 0 0 -chrV 354850 355320 peak_284 0 . 354860 355240 0 1 380 10 0 0 0 -chrV 362350 362630 peak_285 0 . 362380 362620 0 1 240 30 0 0 0 -chrV 386910 387460 peak_286 0 . 386920 387100 0 1 180 10 0 0 0 -chrV 395640 396680 peak_287 0 . 396310 396660 0 1 350 670 0 0 0 -chrV 399330 401380 peak_288 0 . 400760 401100 0 1 340 1430 0 0 0 -chrV 422470 423170 peak_289 0 . 422550 422800 0 1 250 80 0 0 0 -chrV 434410 434690 peak_290 0 . 434420 434680 0 1 260 10 0 0 0 -chrV 435520 435890 peak_291 0 . 435580 435790 0 1 210 60 0 0 0 -chrV 438780 439770 peak_292 0 . 439290 439400 0 1 110 510 0 0 0 -chrV 441600 442770 peak_293 0 . 441890 442530 0 1 640 290 0 0 0 -chrV 448080 449030 peak_294 0 . 448540 448990 0 1 450 460 0 0 0 -chrV 461150 462850 peak_295 0 . 461530 462470 0 2 740,140 380,1180 0 0 0 -chrV 473210 475830 peak_296 0 . 473500 475070 0 2 310,990 290,870 0 0 0 -chrV 490400 491430 peak_297 0 . 490950 491400 0 1 450 550 0 0 0 -chrV 492250 492490 peak_298 0 . 492280 492430 0 1 150 30 0 0 0 -chrV 497370 498140 peak_299 0 . 497680 497920 0 1 240 310 0 0 0 -chrV 544450 545350 peak_300 0 . 544620 545340 0 1 720 170 0 0 0 -chrV 546550 548050 peak_301 0 . 546620 546980 0 1 360 70 0 0 0 -chrV 551170 551440 peak_302 0 . 551230 551430 0 1 200 60 0 0 0 -chrV 569690 570730 peak_303 0 . 569700 570640 0 1 940 10 0 0 0 -chrVI 4280 5300 peak_304 0 . 4340 5290 0 1 950 60 0 0 0 -chrVI 74640 75670 peak_305 0 . 74710 75000 0 1 290 70 0 0 0 -chrVI 82030 82450 peak_306 0 . 82090 82390 0 1 300 60 0 0 0 -chrVI 90020 90930 peak_307 0 . 90340 90900 0 1 560 320 0 0 0 -chrVI 95130 96030 peak_308 0 . 95180 95930 0 1 750 50 0 0 0 -chrVI 101380 101520 peak_309 0 . 101390 101500 0 1 110 10 0 0 0 -chrVI 106290 106750 peak_310 0 . 106320 106740 0 1 420 30 0 0 0 -chrVI 157850 158080 peak_311 0 . 157880 158070 0 1 190 30 0 0 0 -chrVI 176580 177220 peak_312 0 . 176710 176900 0 1 190 130 0 0 0 -chrVI 183020 185320 peak_313 0 . 183030 184780 0 1 1750 10 0 0 0 -chrVI 191100 191720 peak_314 0 . 191470 191690 0 1 220 370 0 0 0 -chrVI 210280 211740 peak_315 0 . 210660 210820 0 1 160 380 0 0 0 -chrVI 220870 221970 peak_316 0 . 221430 221950 0 1 520 560 0 0 0 -chrVI 224400 226590 peak_317 0 . 224490 226480 0 1 1990 90 0 0 0 -chrVI 226680 227440 peak_318 0 . 226720 226860 0 1 140 40 0 0 0 -chrVII 22850 23770 peak_319 0 . 23350 23750 0 1 400 500 0 0 0 -chrVII 54400 57140 peak_320 0 . 56200 56500 0 1 300 1800 0 0 0 -chrVII 73840 74710 peak_321 0 . 73870 74640 0 2 270,310 30,490 0 0 0 -chrVII 76490 76700 peak_322 0 . 76570 76690 0 1 120 80 0 0 0 -chrVII 80770 82330 peak_323 0 . 81240 81370 0 1 130 470 0 0 0 -chrVII 94910 95680 peak_324 0 . 95310 95530 0 1 220 400 0 0 0 -chrVII 110350 110780 peak_325 0 . 110550 110680 0 1 130 200 0 0 0 -chrVII 115400 115910 peak_326 0 . 115410 115850 0 1 440 10 0 0 0 -chrVII 122040 122520 peak_327 0 . 122180 122430 0 1 250 140 0 0 0 -chrVII 124000 124550 peak_328 0 . 124300 124430 0 1 130 300 0 0 0 -chrVII 148960 149420 peak_329 0 . 149080 149380 0 1 300 120 0 0 0 -chrVII 165740 166440 peak_330 0 . 166020 166190 0 1 170 280 0 0 0 -chrVII 190850 191730 peak_331 0 . 190920 191080 0 1 160 70 0 0 0 -chrVII 196680 197550 peak_332 0 . 197130 197470 0 1 340 450 0 0 0 -chrVII 205450 206030 peak_333 0 . 205530 205670 0 1 140 80 0 0 0 -chrVII 206400 206670 peak_334 0 . 206450 206650 0 1 200 50 0 0 0 -chrVII 254220 255250 peak_335 0 . 254340 254480 0 1 140 120 0 0 0 -chrVII 270220 272250 peak_336 0 . 270880 272230 0 1 1350 660 0 0 0 -chrVII 272440 273590 peak_337 0 . 272720 273180 0 1 460 280 0 0 0 -chrVII 276380 277560 peak_338 0 . 277120 277420 0 1 300 740 0 0 0 -chrVII 287040 287530 peak_339 0 . 287280 287520 0 1 240 240 0 0 0 -chrVII 305970 307060 peak_340 0 . 306350 306620 0 1 270 380 0 0 0 -chrVII 310610 310860 peak_341 0 . 310620 310840 0 1 220 10 0 0 0 -chrVII 319630 320030 peak_342 0 . 319700 319940 0 1 240 70 0 0 0 -chrVII 324630 325050 peak_343 0 . 324730 324880 0 1 150 100 0 0 0 -chrVII 345600 346070 peak_344 0 . 345640 345790 0 1 150 40 0 0 0 -chrVII 370890 371640 peak_345 0 . 371200 371590 0 1 390 310 0 0 0 -chrVII 383400 385190 peak_346 0 . 384270 385040 0 1 770 870 0 0 0 -chrVII 401360 401700 peak_347 0 . 401430 401680 0 1 250 70 0 0 0 -chrVII 405330 406000 peak_348 0 . 405490 405650 0 1 160 160 0 0 0 -chrVII 412810 413580 peak_349 0 . 413250 413490 0 1 240 440 0 0 0 -chrVII 423010 423490 peak_350 0 . 423100 423320 0 1 220 90 0 0 0 -chrVII 428120 428400 peak_351 0 . 428140 428390 0 1 250 20 0 0 0 -chrVII 438310 438810 peak_352 0 . 438500 438770 0 1 270 190 0 0 0 -chrVII 483070 484070 peak_353 0 . 483270 483890 0 1 620 200 0 0 0 -chrVII 515780 516550 peak_354 0 . 516150 516520 0 1 370 370 0 0 0 -chrVII 527710 529080 peak_355 0 . 528320 528870 0 2 140,240 610,920 0 0 0 -chrVII 536030 537000 peak_356 0 . 536120 536480 0 1 360 90 0 0 0 -chrVII 546470 547870 peak_357 0 . 546950 547670 0 1 720 480 0 0 0 -chrVII 555450 555960 peak_358 0 . 555480 555670 0 1 190 30 0 0 0 -chrVII 559920 560470 peak_359 0 . 560130 560450 0 1 320 210 0 0 0 -chrVII 566770 567490 peak_360 0 . 567060 567310 0 1 250 290 0 0 0 -chrVII 576430 576910 peak_361 0 . 576610 576800 0 1 190 180 0 0 0 -chrVII 581610 581920 peak_362 0 . 581790 581910 0 1 120 180 0 0 0 -chrVII 592090 593240 peak_363 0 . 592510 593110 0 1 600 420 0 0 0 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82390 0 1 300 60 0 0 0 -chrVI 90020 90930 peak_307 0 . 90340 90900 0 1 560 320 0 0 0 -chrVI 95130 96030 peak_308 0 . 95180 95930 0 1 750 50 0 0 0 -chrVI 101380 101520 peak_309 0 . 101390 101500 0 1 110 10 0 0 0 -chrVI 106290 106750 peak_310 0 . 106320 106740 0 1 420 30 0 0 0 -chrVI 157850 158080 peak_311 0 . 157880 158070 0 1 190 30 0 0 0 -chrVI 176580 177220 peak_312 0 . 176710 176900 0 1 190 130 0 0 0 -chrVI 183020 185320 peak_313 0 . 183030 184780 0 1 1750 10 0 0 0 -chrVI 191100 191720 peak_314 0 . 191470 191690 0 1 220 370 0 0 0 -chrVI 210280 211740 peak_315 0 . 210660 210820 0 1 160 380 0 0 0 -chrVI 220870 221970 peak_316 0 . 221430 221950 0 1 520 560 0 0 0 -chrVI 224400 226590 peak_317 0 . 224490 226480 0 1 1990 90 0 0 0 -chrVI 226680 227440 peak_318 0 . 226720 226860 0 1 140 40 0 0 0 -chrVII 22850 23770 peak_319 0 . 23350 23750 0 1 400 500 0 0 0 -chrVII 54400 57140 peak_320 0 . 56200 56500 0 1 300 1800 0 0 0 -chrVII 73840 74710 peak_321 0 . 73870 74640 0 2 270,310 30,490 0 0 0 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peak_924 0 . 900820 901020 0 1 200 170 0 0 0 -chrXVI 921030 921430 peak_925 0 . 921220 921370 0 1 150 190 0 0 0 -chrXVI 927420 928440 peak_926 0 . 927870 928180 0 1 310 450 0 0 0 -chrXVI 942500 942840 peak_927 0 . 942550 942750 0 1 200 50 0 0 0 -chrXVI 947790 948010 peak_928 0 . 947870 948000 0 1 130 80 0 0 0 From dd2dfbd15df29e6051dfca14db1dc6f0db3d3e66 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Fri, 15 Mar 2024 11:31:38 -0400 Subject: [PATCH 5/7] add --hmm-type option for poisson or gaussian hmm --- MACS3/Commands/hmmratac_cmd.py | 74 +- MACS3/Signal/HMMR_HMM.pyx | 73 +- MACS3/Signal/HMMR_Signal_Processing.pyx | 44 +- MACS3/Utilities/OptValidator.py | 4 + bin/macs3 | 2 + test/cmdlinetest | 4 +- ...ac_yeast500k_accessible_regions.gappedPeak | 929 ++++++++++++++++++ 7 files changed, 1042 insertions(+), 88 deletions(-) create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak diff --git a/MACS3/Commands/hmmratac_cmd.py b/MACS3/Commands/hmmratac_cmd.py index 03008b3f..524afd14 100644 --- a/MACS3/Commands/hmmratac_cmd.py +++ b/MACS3/Commands/hmmratac_cmd.py @@ -29,8 +29,7 @@ from MACS3.IO.Parser import BAMPEParser, BEDPEParser #BAMaccessor from MACS3.Signal.HMMR_EM import HMMR_EM from MACS3.Signal.HMMR_Signal_Processing import generate_weight_mapping, generate_digested_signals, extract_signals_from_regions -# from MACS3.Signal.HMMR_HMM import hmm_model_init, hmm_model_save -# from MACS3.Signal.HMMR_HMM import hmm_training, hmm_predict, hmm_model_init, hmm_model_save +from MACS3.Signal.HMMR_HMM import hmm_training, hmm_predict, hmm_model_init, hmm_model_save from MACS3.Signal.Region import Regions from MACS3.Signal.BedGraph import bedGraphTrackI @@ -43,38 +42,7 @@ # ------------------------------------ # Misc functions # ------------------------------------ -import hmmlearn -from hmmlearn.hmm import PoissonHMM -def hmm_training (training_data, training_data_lengths, n_states = 3, random_seed = 12345): - rs = np.random.RandomState(np.random.MT19937(np.random.SeedSequence(random_seed))) - hmm_model = PoissonHMM( n_components= n_states, random_state = rs, verbose = False ) - hmm_model = hmm_model.fit( training_data, training_data_lengths ) - assert hmm_model.n_features == 4 - return hmm_model - -def hmm_predict( signals, lens, hmm_model ): - predictions = hmm_model.predict_proba( signals, lens ) - return predictions - -def hmm_model_save( model_file, hmm_model, hmm_binsize, i_open_region, i_nucleosomal_region, i_background_region ): - with open( model_file, "w" ) as f: - json.dump( {"startprob":hmm_model.startprob_.tolist(), - "transmat":hmm_model.transmat_.tolist(), - "lambdas":hmm_model.lambdas_.tolist(), - "n_features":int(hmm_model.n_features), - "i_open_region":int(i_open_region), - "i_background_region":int(i_background_region), - "i_nucleosomal_region":int(i_nucleosomal_region), - "hmm_binsize":int(hmm_binsize)}, f ) - -def hmm_model_init( model_file ): - with open( model_file ) as f: - m = json.load( f ) - hmm_model = PoissonHMM( n_components=3) - hmm_model.startprob_ = np.array(m["startprob"]) - hmm_model.transmat_ = np.array(m["transmat"]) - hmm_model.n_features = m["n_features"] - return [ hmm_model, m["i_open_region"], m["i_background_region"], m["i_nucleosomal_region"], m["hmm_binsize"] ] + # ------------------------------------ # Main function # ------------------------------------ @@ -306,15 +274,16 @@ def run( args ): # 4. Train HMM ############################################# # if model file is provided, we skip this step + # include options.hmm_type and make it backwards compatible, if no hmm_type default is gaussian if options.hmm_file: options.info( f"#4 Load Hidden Markov Model from given model file") - hmm_model, i_open_region, i_background_region, i_nucleosomal_region, options.hmm_binsize = hmm_model_init( options.hmm_file ) + hmm_model, i_open_region, i_background_region, i_nucleosomal_region, options.hmm_binsize, options.hmm_type = hmm_model_init( options.hmm_file ) else: options.info( f"#4 Train Hidden Markov Model with Multivariate Gaussian Emission" ) # extract signals within peak using the given binsize options.info( f"# Extract signals in training regions with bin size of {options.hmm_binsize}") - [ training_bins, training_data, training_data_lengths ] = extract_signals_from_regions( digested_atac_signals, training_regions, binsize = options.hmm_binsize ) + [ training_bins, training_data, training_data_lengths ] = extract_signals_from_regions( digested_atac_signals, training_regions, binsize = options.hmm_binsize, hmm_type = options.hmm_type ) if options.save_train: f = open( training_datafile, "w" ) @@ -331,25 +300,28 @@ def run( args ): options.info( f"# Use Baum-Welch algorithm to train the HMM") - hmm_model = hmm_training( training_data, training_data_lengths, random_seed = options.hmm_randomSeed, ) + hmm_model = hmm_training( training_data, training_data_lengths, random_seed = options.hmm_randomSeed, hmm_type = options.hmm_type ) options.info( f"# HMM converged: {hmm_model.monitor_.converged}") # label hidden states - lambdas_sum = np.sum( hmm_model.lambdas_, axis=1 ) + if options.hmm_type == "gaussian": + means_sum = np.sum( hmm_model.means_, axis=1 ) + if options.hmm_type == "poisson": + means_sum = np.sum( hmm_model.lambdas_, axis=1 ) # first, the state with the highest overall emission is the open state - i_open_region = np.where( lambdas_sum == max(lambdas_sum) )[0][0] + i_open_region = np.where( means_sum == max(means_sum) )[0][0] # second, the state with lowest overall emission is the bg state - i_background_region = np.where( lambdas_sum == min(lambdas_sum) )[0][0] + i_background_region = np.where( means_sum == min(means_sum) )[0][0] # last one is the nuc state (note it may not be accurate though i_nucleosomal_region = list(set([0, 1, 2]) - set([i_open_region, i_background_region]))[0] # write hmm into model file options.info( f"# Write HMM parameters into JSON: {hmm_modelfile}") - hmm_model_save( hmm_modelfile, hmm_model, options.hmm_binsize, i_open_region, i_nucleosomal_region, i_background_region ) + hmm_model_save( hmm_modelfile, hmm_model, options.hmm_binsize, i_open_region, i_nucleosomal_region, i_background_region, options.hmm_type ) # if --modelonly option provided, exit script after hmm model is saved if options.hmm_modelonly: @@ -374,11 +346,19 @@ def run( args ): options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[0], hmm_model.transmat_[0]) ) options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[1], hmm_model.transmat_[1]) ) options.info( "# {0:>10s}-> {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g}".format(assignments[2], hmm_model.transmat_[2]) ) - options.info( f"# HMM Emissions (lambdas): ") - options.info( "# {0[0]:>10s} {0[1]:>10s} {0[2]:>10s} {0[3]:>10s}".format( ["short", "mono", "di", "tri"] ) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[0], hmm_model.lambdas_[0]) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[1], hmm_model.lambdas_[1]) ) - options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[2], hmm_model.lambdas_[2]) ) + + if options.hmm_type == 'gaussian': + options.info( f"# HMM Emissions (means): ") + options.info( "# {0[0]:>10s} {0[1]:>10s} {0[2]:>10s} {0[3]:>10s}".format( ["short", "mono", "di", "tri"] ) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[0], hmm_model.means_[0]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[1], hmm_model.means_[1]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[2], hmm_model.means_[2]) ) + if options.hmm_type == 'poisson': + options.info( f"# HMM Emissions (lambdas): ") + options.info( "# {0[0]:>10s} {0[1]:>10s} {0[2]:>10s} {0[3]:>10s}".format( ["short", "mono", "di", "tri"] ) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[0], hmm_model.lambdas_[0]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[1], hmm_model.lambdas_[1]) ) + options.info( "# {0:>10s}: {1[0]:>10.4g} {1[1]:>10.4g} {1[2]:>10.4g} {1[3]:>10.4g}".format(assignments[2], hmm_model.lambdas_[2]) ) ############################################# @@ -418,7 +398,7 @@ def run( args ): n += 1 cr = candidate_regions.pop( options.decoding_steps ) options.info( "# decoding %d..." % ( n*options.decoding_steps ) ) - [ cr_bins, cr_data, cr_data_lengths ] = extract_signals_from_regions( digested_atac_signals, cr, binsize = options.hmm_binsize ) + [ cr_bins, cr_data, cr_data_lengths ] = extract_signals_from_regions( digested_atac_signals, cr, binsize = options.hmm_binsize, hmm_type = options.hmm_type ) #options.info( "# extracted signals in the candidate regions") candidate_bins.extend( cr_bins ) #options.info( "# saving information regarding the candidate regions") diff --git a/MACS3/Signal/HMMR_HMM.pyx b/MACS3/Signal/HMMR_HMM.pyx index 6444a101..9b27ae90 100644 --- a/MACS3/Signal/HMMR_HMM.pyx +++ b/MACS3/Signal/HMMR_HMM.pyx @@ -21,7 +21,7 @@ import numpy as np cimport numpy as np from cpython cimport bool import hmmlearn -from hmmlearn.hmm import GaussianHMM +from hmmlearn.hmm import GaussianHMM, PoissonHMM from sklearn import cluster import json # from hmmlearn cimport hmm @@ -57,13 +57,16 @@ cdef inline float get_weighted_density( int x, float m, float v, w ): # ------------------------------------ -cpdef hmm_training( list training_data, list training_data_lengths, int n_states = 3, int random_seed = 12345, covar = 'full' ): +cpdef hmm_training( list training_data, list training_data_lengths, int n_states = 3, int random_seed = 12345, covar = 'full', hmm_type = 'gaussian' ): # training data should be in array like format: X = np.array([[.5, .3, .1, .1], [.6, .4, 0, 0]]) # if we do not want init_prob to be updated through learning, set params = 'tmc' and init_prob = initial_state otherwise it will be overwritten # according to base documentation, if init_prob not stated, it is set to be equally likely for any state (1/ # of components) # if we have other known parameters, we should set these (ie: means_weights, covariance_type etc.) rs = np.random.RandomState(np.random.MT19937(np.random.SeedSequence(random_seed))) - hmm_model = GaussianHMM( n_components= n_states, covariance_type = covar, random_state = rs, verbose = False ) + if hmm_type == "gaussian": + hmm_model = GaussianHMM( n_components= n_states, covariance_type = covar, random_state = rs, verbose = False ) + if hmm_type == "poisson": + hmm_model = PoissonHMM( n_components= n_states, random_state = rs, verbose = False ) hmm_model = hmm_model.fit( training_data, training_data_lengths ) assert hmm_model.n_features == 4 return hmm_model @@ -72,33 +75,51 @@ cpdef hmm_predict( list signals, list lens, hmm_model ): predictions = hmm_model.predict_proba( signals, lens ) return predictions -cpdef void hmm_model_save( str model_file, object hmm_model, int hmm_binsize, int i_open_region, int i_nucleosomal_region, int i_background_region ): - if hmm_model.covariance_type == "diag": - covars = hmm_model.covars_.diagonal(axis1=1, axis2=2) - elif hmm_model.covariance_type == "full": - covars = hmm_model.covars_ - else: - raise Exception(f"Unknown covariance type {hmm_model.covariance_type}") - with open( model_file, "w" ) as f: - json.dump( {"startprob":hmm_model.startprob_.tolist(), - "transmat":hmm_model.transmat_.tolist(), - "means":hmm_model.means_.tolist(), - "covars":covars.tolist(), - "covariance_type":hmm_model.covariance_type, - "n_features":int(hmm_model.n_features), - "i_open_region":int(i_open_region), - "i_background_region":int(i_background_region), - "i_nucleosomal_region":int(i_nucleosomal_region), - "hmm_binsize":int(hmm_binsize)}, f ) +cpdef void hmm_model_save( str model_file, object hmm_model, int hmm_binsize, int i_open_region, int i_nucleosomal_region, int i_background_region, hmm_type ): + if hmm_type == "gaussian": + if hmm_model.covariance_type == "diag": + covars = hmm_model.covars_.diagonal(axis1=1, axis2=2) + elif hmm_model.covariance_type == "full": + covars = hmm_model.covars_ + else: + raise Exception(f"Unknown covariance type {hmm_model.covariance_type}") + with open( model_file, "w" ) as f: + json.dump( {"startprob":hmm_model.startprob_.tolist(), + "transmat":hmm_model.transmat_.tolist(), + "means":hmm_model.means_.tolist(), + "covars":covars.tolist(), + "covariance_type":hmm_model.covariance_type, + "n_features":int(hmm_model.n_features), + "i_open_region":int(i_open_region), + "i_background_region":int(i_background_region), + "i_nucleosomal_region":int(i_nucleosomal_region), + "hmm_binsize":int(hmm_binsize), + "hmm_type":hmm_type}, f ) + if hmm_type == "poisson": + with open( model_file, "w" ) as f: + json.dump( {"startprob":hmm_model.startprob_.tolist(), + "transmat":hmm_model.transmat_.tolist(), + "lambdas":hmm_model.lambdas_.tolist(), + "n_features":int(hmm_model.n_features), + "i_open_region":int(i_open_region), + "i_background_region":int(i_background_region), + "i_nucleosomal_region":int(i_nucleosomal_region), + "hmm_binsize":int(hmm_binsize), + "hmm_type":hmm_type}, f ) cpdef list hmm_model_init( str model_file ): with open( model_file ) as f: m = json.load( f ) - hmm_model = GaussianHMM( n_components=3, covariance_type=m["covariance_type"] ) + hmm_type = m.get("hmm_type", "gaussian") #if no model_type written, assume older file and use gaussian + if hmm_type == "gaussian": + hmm_model = GaussianHMM( n_components=3, covariance_type=m["covariance_type"] ) + hmm_model.means_ = np.array(m["means"]) + hmm_model.covars_ = np.array(m["covars"]) + hmm_model.covariance_type = m["covariance_type"] + if hmm_type == "poisson": + hmm_model = PoissonHMM( n_components=3 ) + hmm_model.lambdas_ = np.array(m["lambdas"]) hmm_model.startprob_ = np.array(m["startprob"]) hmm_model.transmat_ = np.array(m["transmat"]) - hmm_model.means_ = np.array(m["means"]) - hmm_model.covars_ = np.array(m["covars"]) - hmm_model.covariance_type = m["covariance_type"] hmm_model.n_features = m["n_features"] - return [ hmm_model, m["i_open_region"], m["i_background_region"], m["i_nucleosomal_region"], m["hmm_binsize"] ] + return [ hmm_model, m["i_open_region"], m["i_background_region"], m["i_nucleosomal_region"], m["hmm_binsize"], hmm_type ] diff --git a/MACS3/Signal/HMMR_Signal_Processing.pyx b/MACS3/Signal/HMMR_Signal_Processing.pyx index 75b39a7f..249477f1 100644 --- a/MACS3/Signal/HMMR_Signal_Processing.pyx +++ b/MACS3/Signal/HMMR_Signal_Processing.pyx @@ -130,7 +130,7 @@ cpdef list generate_digested_signals( object petrack, list weight_mapping ): ret_bedgraphs.append( bdg ) return ret_bedgraphs -cpdef list extract_signals_from_regions( list signals, object regions, int binsize = 10 ): +cpdef list extract_signals_from_regions( list signals, object regions, int binsize = 10, hmm_type = 'gaussian' ): # we will take regions in peaks, create a bedGraphTrackI with # binned regions in peaks, then let them overlap with signals to # create a list (4) of value arrays. @@ -168,19 +168,35 @@ cpdef list extract_signals_from_regions( list signals, object regions, int binsi counter = 0 prev_c = extracted_len[0][0] c = 0 - for i in range( nn ): - ret_training_bins.append( extracted_positions[0][i] ) - ret_training_data.append( - [ int(max( 0.0001, extracted_data[0][i] )), - int(max( 0.0001, extracted_data[1][i] )), - int(max( 0.0001, extracted_data[2][i] )), - int(max( 0.0001, extracted_data[3][i] )) ] ) - c = extracted_len[0][i] - if counter != 0 and c != prev_c: - ret_training_lengths.append( counter ) - counter = 0 - prev_c = c - counter += 1 + if hmm_type == "gaussian": + for i in range( nn ): + ret_training_bins.append( extracted_positions[0][i] ) + ret_training_data.append( + [max( 0.0001, extracted_data[0][i] ), + max( 0.0001, extracted_data[1][i] ), + max( 0.0001, extracted_data[2][i] ), + max( 0.0001, extracted_data[3][i] ) ] ) + c = extracted_len[0][i] + if counter != 0 and c != prev_c: + ret_training_lengths.append( counter ) + counter = 0 + prev_c = c + counter += 1 + #poisson can only take int values as input + if hmm_type == "poisson": + for i in range( nn ): + ret_training_bins.append( extracted_positions[0][i] ) + ret_training_data.append( + [ int(max( 0.0001, extracted_data[0][i] )), + int(max( 0.0001, extracted_data[1][i] )), + int(max( 0.0001, extracted_data[2][i] )), + int(max( 0.0001, extracted_data[3][i] )) ] ) + c = extracted_len[0][i] + if counter != 0 and c != prev_c: + ret_training_lengths.append( counter ) + counter = 0 + prev_c = c + counter += 1 # last region ret_training_lengths.append( counter ) assert sum(ret_training_lengths) == len(ret_training_data) diff --git a/MACS3/Utilities/OptValidator.py b/MACS3/Utilities/OptValidator.py index f44a6f23..b317ce27 100644 --- a/MACS3/Utilities/OptValidator.py +++ b/MACS3/Utilities/OptValidator.py @@ -870,6 +870,10 @@ def opt_validate_hmmratac ( options ): if options.hmm_modelonly: options.argtxt += "# Program will stop after generating model, which can be later applied with '--model'. \n" + # hmm_modelType + if options.hmm_type: + options.argtxt += "# Use --hmm-type to select a Gaussian ('gaussian') or Poisson ('poisson') model for the hidden markov model in HMMRATAC. Default: 'gaussian'. \n" + # Peak Calling if options.prescan_cutoff <= 1: diff --git a/bin/macs3 b/bin/macs3 index d93414f7..7b8c442a 100644 --- a/bin/macs3 +++ b/bin/macs3 @@ -973,6 +973,8 @@ plus an extra option for the HMM model file like `macs3 hmmratac help = "A JSON file generated from previous HMMRATAC run to use instead of creating new one. When provided, HMM training will be skipped. Default: NA" ) group_hmm.add_argument( "--modelonly", dest = "hmm_modelonly", action = "store_true", default = False, help = "Stop the program after generating model. Use this option to generate HMM model ONLY, which can be later applied with `--model`. Default: False") + group_hmm.add_argument( "--hmm-type", dest = "hmm_type", type = str, choices = ("gaussian", "poisson"), default = "gaussian", + help = "Use --hmm-type to select a Gaussian ('gaussian') or Poisson ('poisson') model for the hidden markov model in HMMRATAC. Default: 'gaussian'.") # group for peak calling arguments group_call = argparser_hmmratac.add_argument_group( "Peak calling/HMM decoding arguments" ) diff --git a/test/cmdlinetest b/test/cmdlinetest index b554bc3a..3841e3cb 100755 --- a/test/cmdlinetest +++ b/test/cmdlinetest @@ -213,7 +213,9 @@ macs3 randsample -i $CHIPCONTIGS50K -n 100000 --seed 31415926 --outdir ${OUTPUTD echo "15. hmmratac" mkdir ${OUTPUTDIR_PREFIX}_run_hmmratac -macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_poisson --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM --hmm-type poisson -n hmmratac_yeast500k_poisson --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM --hmm-type gaussian -n hmmratac_yeast500k --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log # macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log # macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak new file mode 100644 index 00000000..a877edea --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak @@ -0,0 +1,929 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 +chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 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303750 0 1 150 170 0 0 0 +chrV 305910 306270 peak_279 0 . 305940 306260 0 1 320 30 0 0 0 +chrV 311180 312180 peak_280 0 . 311510 312160 0 1 650 330 0 0 0 +chrV 334590 336790 peak_281 0 . 335420 335980 0 1 560 830 0 0 0 +chrV 342470 343100 peak_282 0 . 342480 342800 0 1 320 10 0 0 0 +chrV 349130 350180 peak_283 0 . 349450 349850 0 1 400 320 0 0 0 +chrV 354850 355320 peak_284 0 . 354860 355240 0 1 380 10 0 0 0 +chrV 362350 362630 peak_285 0 . 362380 362620 0 1 240 30 0 0 0 +chrV 386910 387460 peak_286 0 . 386920 387100 0 1 180 10 0 0 0 +chrV 395640 396680 peak_287 0 . 396310 396660 0 1 350 670 0 0 0 +chrV 399330 401380 peak_288 0 . 400760 401100 0 1 340 1430 0 0 0 +chrV 422470 423170 peak_289 0 . 422550 422800 0 1 250 80 0 0 0 +chrV 434410 434690 peak_290 0 . 434420 434680 0 1 260 10 0 0 0 +chrV 435520 435890 peak_291 0 . 435580 435790 0 1 210 60 0 0 0 +chrV 438780 439770 peak_292 0 . 439290 439400 0 1 110 510 0 0 0 +chrV 441600 442770 peak_293 0 . 441890 442530 0 1 640 290 0 0 0 +chrV 448080 449030 peak_294 0 . 448540 448990 0 1 450 460 0 0 0 +chrV 461150 462850 peak_295 0 . 461530 462470 0 2 740,140 380,1180 0 0 0 +chrV 473210 475830 peak_296 0 . 473500 475070 0 2 310,990 290,870 0 0 0 +chrV 490400 491430 peak_297 0 . 490950 491400 0 1 450 550 0 0 0 +chrV 492250 492490 peak_298 0 . 492280 492430 0 1 150 30 0 0 0 +chrV 497370 498140 peak_299 0 . 497680 497920 0 1 240 310 0 0 0 +chrV 544450 545350 peak_300 0 . 544620 545340 0 1 720 170 0 0 0 +chrV 546550 548050 peak_301 0 . 546620 546980 0 1 360 70 0 0 0 +chrV 551170 551440 peak_302 0 . 551230 551430 0 1 200 60 0 0 0 +chrV 569690 570730 peak_303 0 . 569700 570640 0 1 940 10 0 0 0 +chrVI 4280 5300 peak_304 0 . 4340 5290 0 1 950 60 0 0 0 +chrVI 74640 75670 peak_305 0 . 74710 75000 0 1 290 70 0 0 0 +chrVI 82030 82450 peak_306 0 . 82090 82390 0 1 300 60 0 0 0 +chrVI 90020 90930 peak_307 0 . 90340 90900 0 1 560 320 0 0 0 +chrVI 95130 96030 peak_308 0 . 95180 95930 0 1 750 50 0 0 0 +chrVI 101380 101520 peak_309 0 . 101390 101500 0 1 110 10 0 0 0 +chrVI 106290 106750 peak_310 0 . 106320 106740 0 1 420 30 0 0 0 +chrVI 157850 158080 peak_311 0 . 157880 158070 0 1 190 30 0 0 0 +chrVI 176580 177220 peak_312 0 . 176710 176900 0 1 190 130 0 0 0 +chrVI 183020 185320 peak_313 0 . 183030 184780 0 1 1750 10 0 0 0 +chrVI 191100 191720 peak_314 0 . 191470 191690 0 1 220 370 0 0 0 +chrVI 210280 211740 peak_315 0 . 210660 210820 0 1 160 380 0 0 0 +chrVI 220870 221970 peak_316 0 . 221430 221950 0 1 520 560 0 0 0 +chrVI 224400 226590 peak_317 0 . 224490 226480 0 1 1990 90 0 0 0 +chrVI 226680 227440 peak_318 0 . 226720 226860 0 1 140 40 0 0 0 +chrVII 22850 23770 peak_319 0 . 23350 23750 0 1 400 500 0 0 0 +chrVII 54400 57140 peak_320 0 . 56200 56500 0 1 300 1800 0 0 0 +chrVII 73840 74710 peak_321 0 . 73870 74640 0 2 270,310 30,490 0 0 0 +chrVII 76490 76700 peak_322 0 . 76570 76690 0 1 120 80 0 0 0 +chrVII 80770 82330 peak_323 0 . 81240 81370 0 1 130 470 0 0 0 +chrVII 94910 95680 peak_324 0 . 95310 95530 0 1 220 400 0 0 0 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0 +chrVII 305970 307060 peak_340 0 . 306350 306620 0 1 270 380 0 0 0 +chrVII 310610 310860 peak_341 0 . 310620 310840 0 1 220 10 0 0 0 +chrVII 319630 320030 peak_342 0 . 319700 319940 0 1 240 70 0 0 0 +chrVII 324630 325050 peak_343 0 . 324730 324880 0 1 150 100 0 0 0 +chrVII 345600 346070 peak_344 0 . 345640 345790 0 1 150 40 0 0 0 +chrVII 370890 371640 peak_345 0 . 371200 371590 0 1 390 310 0 0 0 +chrVII 383400 385190 peak_346 0 . 384270 385040 0 1 770 870 0 0 0 +chrVII 401360 401700 peak_347 0 . 401430 401680 0 1 250 70 0 0 0 +chrVII 405330 406000 peak_348 0 . 405490 405650 0 1 160 160 0 0 0 +chrVII 412810 413580 peak_349 0 . 413250 413490 0 1 240 440 0 0 0 +chrVII 423010 423490 peak_350 0 . 423100 423320 0 1 220 90 0 0 0 +chrVII 428120 428400 peak_351 0 . 428140 428390 0 1 250 20 0 0 0 +chrVII 438310 438810 peak_352 0 . 438500 438770 0 1 270 190 0 0 0 +chrVII 483070 484070 peak_353 0 . 483270 483890 0 1 620 200 0 0 0 +chrVII 515780 516550 peak_354 0 . 516150 516520 0 1 370 370 0 0 0 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250 0 0 0 +chrVII 661700 661990 peak_370 0 . 661770 661980 0 1 210 70 0 0 0 +chrVII 700570 701110 peak_371 0 . 700670 701030 0 1 360 100 0 0 0 +chrVII 702690 703270 peak_372 0 . 702900 703030 0 1 130 210 0 0 0 +chrVII 706810 707310 peak_373 0 . 707080 707240 0 1 160 270 0 0 0 +chrVII 726640 726920 peak_374 0 . 726650 726870 0 1 220 10 0 0 0 +chrVII 731690 733080 peak_375 0 . 731980 732110 0 1 130 290 0 0 0 +chrVII 753650 754550 peak_376 0 . 753970 754140 0 1 170 320 0 0 0 +chrVII 756080 757400 peak_377 0 . 756560 756810 0 1 250 480 0 0 0 +chrVII 765940 766830 peak_378 0 . 766000 766600 0 1 600 60 0 0 0 +chrVII 771800 772340 peak_379 0 . 772000 772280 0 1 280 200 0 0 0 +chrVII 774270 774850 peak_380 0 . 774370 774830 0 1 460 100 0 0 0 +chrVII 785350 787170 peak_381 0 . 785500 787140 0 2 730,110 150,1680 0 0 0 +chrVII 788060 788810 peak_382 0 . 788280 788670 0 1 390 220 0 0 0 +chrVII 809530 810740 peak_383 0 . 809660 810140 0 1 480 130 0 0 0 +chrVII 810880 811390 peak_384 0 . 810920 811050 0 1 130 40 0 0 0 +chrVII 811700 812470 peak_385 0 . 812010 812140 0 1 130 310 0 0 0 +chrVII 821450 823020 peak_386 0 . 822580 822860 0 1 280 1130 0 0 0 +chrVII 845570 845750 peak_387 0 . 845580 845740 0 1 160 10 0 0 0 +chrVII 856410 858130 peak_388 0 . 856460 857720 0 2 820,310 50,1000 0 0 0 +chrVII 859070 859760 peak_389 0 . 859460 859710 0 1 250 390 0 0 0 +chrVII 882840 883820 peak_390 0 . 882890 883690 0 1 800 50 0 0 0 +chrVII 883920 884410 peak_391 0 . 883970 884380 0 1 410 50 0 0 0 +chrVII 904940 905820 peak_392 0 . 905010 905810 0 1 800 70 0 0 0 +chrVII 912960 915600 peak_393 0 . 914760 915150 0 2 110,190 1800,2000 0 0 0 +chrVII 920120 920640 peak_394 0 . 920130 920540 0 1 410 10 0 0 0 +chrVII 974260 974770 peak_395 0 . 974520 974740 0 1 220 260 0 0 0 +chrVII 976750 977190 peak_396 0 . 976850 977100 0 1 250 100 0 0 0 +chrVII 986840 987830 peak_397 0 . 986860 987680 0 2 110,670 20,170 0 0 0 +chrVII 993790 994650 peak_398 0 . 993980 994280 0 1 300 190 0 0 0 +chrVII 1000000 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350 0 0 0 +chrXVI 404020 404710 peak_883 0 . 404360 404670 0 1 310 340 0 0 0 +chrXVI 406240 407000 peak_884 0 . 406320 406510 0 1 190 80 0 0 0 +chrXVI 411790 412320 peak_885 0 . 411960 412270 0 1 310 170 0 0 0 +chrXVI 424990 425960 peak_886 0 . 425200 425760 0 1 560 210 0 0 0 +chrXVI 431500 432430 peak_887 0 . 431700 432310 0 1 610 200 0 0 0 +chrXVI 450730 451390 peak_888 0 . 450790 451220 0 1 430 60 0 0 0 +chrXVI 453390 454000 peak_889 0 . 453450 453860 0 1 410 60 0 0 0 +chrXVI 454260 455300 peak_890 0 . 454380 455220 0 2 300,430 120,530 0 0 0 +chrXVI 463950 464220 peak_891 0 . 463990 464170 0 1 180 40 0 0 0 +chrXVI 481690 482430 peak_892 0 . 482030 482360 0 1 330 340 0 0 0 +chrXVI 497360 497960 peak_893 0 . 497790 497950 0 1 160 430 0 0 0 +chrXVI 502390 503330 peak_894 0 . 502450 503170 0 2 180,440 60,340 0 0 0 +chrXVI 519320 520580 peak_895 0 . 520250 520530 0 1 280 930 0 0 0 +chrXVI 526690 527440 peak_896 0 . 526850 527170 0 1 320 160 0 0 0 +chrXVI 572280 572530 peak_897 0 . 572290 572500 0 1 210 10 0 0 0 +chrXVI 581980 582210 peak_898 0 . 582070 582190 0 1 120 90 0 0 0 +chrXVI 585850 586630 peak_899 0 . 586100 586260 0 1 160 250 0 0 0 +chrXVI 641190 642000 peak_900 0 . 641210 641780 0 1 570 20 0 0 0 +chrXVI 643520 643700 peak_901 0 . 643530 643690 0 1 160 10 0 0 0 +chrXVI 644300 645750 peak_902 0 . 645040 645700 0 1 660 740 0 0 0 +chrXVI 653630 654070 peak_903 0 . 653830 654050 0 1 220 200 0 0 0 +chrXVI 678370 679380 peak_904 0 . 678480 679320 0 1 840 110 0 0 0 +chrXVI 694640 695550 peak_905 0 . 694980 695440 0 1 460 340 0 0 0 +chrXVI 699540 700360 peak_906 0 . 700070 700330 0 1 260 530 0 0 0 +chrXVI 719360 719750 peak_907 0 . 719410 719520 0 1 110 50 0 0 0 +chrXVI 731850 732230 peak_908 0 . 731890 732220 0 1 330 40 0 0 0 +chrXVI 769160 769390 peak_909 0 . 769170 769380 0 1 210 10 0 0 0 +chrXVI 770650 771070 peak_910 0 . 770830 771060 0 1 230 180 0 0 0 +chrXVI 794180 794980 peak_911 0 . 794620 794890 0 1 270 440 0 0 0 +chrXVI 809560 810320 peak_912 0 . 809740 810220 0 1 480 180 0 0 0 +chrXVI 812800 813050 peak_913 0 . 812820 812990 0 1 170 20 0 0 0 +chrXVI 822000 822520 peak_914 0 . 822090 822380 0 1 290 90 0 0 0 +chrXVI 828520 829600 peak_915 0 . 828830 829540 0 1 710 310 0 0 0 +chrXVI 834320 834850 peak_916 0 . 834370 834500 0 1 130 50 0 0 0 +chrXVI 840140 840890 peak_917 0 . 840280 840830 0 2 320,170 140,520 0 0 0 +chrXVI 844600 845320 peak_918 0 . 844900 845230 0 1 330 300 0 0 0 +chrXVI 854940 856320 peak_919 0 . 855770 856190 0 1 420 830 0 0 0 +chrXVI 856800 857210 peak_920 0 . 856820 857170 0 2 190,110 20,260 0 0 0 +chrXVI 860240 860900 peak_921 0 . 860250 860780 0 1 530 10 0 0 0 +chrXVI 880230 880520 peak_922 0 . 880260 880430 0 1 170 30 0 0 0 +chrXVI 899510 900620 peak_923 0 . 899820 900280 0 1 460 310 0 0 0 +chrXVI 900650 901460 peak_924 0 . 900820 901020 0 1 200 170 0 0 0 +chrXVI 921030 921430 peak_925 0 . 921220 921370 0 1 150 190 0 0 0 +chrXVI 927420 928440 peak_926 0 . 927870 928180 0 1 310 450 0 0 0 +chrXVI 942500 942840 peak_927 0 . 942550 942750 0 1 200 50 0 0 0 +chrXVI 947790 948010 peak_928 0 . 947870 948000 0 1 130 80 0 0 0 From 0b99645034b042ed066204bc1bb17ca271506dbc Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Fri, 15 Mar 2024 15:41:16 -0400 Subject: [PATCH 6/7] add back in extra hmmratac cmdline tests --- test/cmdlinetest | 28 +- ...st500k_bedpe_accessible_regions.gappedPeak | 929 ++++++++++++++++++ ...edpe_poisson_accessible_regions.gappedPeak | 729 ++++++++++++++ ...ad_hmm_model_accessible_regions.gappedPeak | 929 ++++++++++++++++++ ...ning_regions_accessible_regions.gappedPeak | 929 ++++++++++++++++++ ...ad_hmm_model_accessible_regions.gappedPeak | 729 ++++++++++++++ ...ning_regions_accessible_regions.gappedPeak | 729 ++++++++++++++ 7 files changed, 4992 insertions(+), 10 deletions(-) create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_poisson_accessible_regions.gappedPeak create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_load_hmm_model_accessible_regions.gappedPeak create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_load_training_regions_accessible_regions.gappedPeak create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_hmm_model_accessible_regions.gappedPeak create mode 100644 test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_training_regions_accessible_regions.gappedPeak diff --git a/test/cmdlinetest b/test/cmdlinetest index 3841e3cb..e0ba4abc 100755 --- a/test/cmdlinetest +++ b/test/cmdlinetest @@ -213,21 +213,29 @@ macs3 randsample -i $CHIPCONTIGS50K -n 100000 --seed 31415926 --outdir ${OUTPUTD echo "15. hmmratac" mkdir ${OUTPUTDIR_PREFIX}_run_hmmratac -macs3 hmmratac -i $ATACSEQBAM --hmm-type poisson -n hmmratac_yeast500k_poisson --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log -macs3 hmmratac -i $ATACSEQBAM --hmm-type gaussian -n hmmratac_yeast500k --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log -macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log -# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +# check default (no hmm-type specified) this is technically the same as --hmm-type gaussian (can include if preferred) +# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log -# macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log +# check both gaussian and poisson hmm models, save training data +macs3 hmmratac -i $ATACSEQBAM --hmm-type gaussian -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM --hmm-type poisson -n hmmratac_yeast500k_poisson --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_poisson.log -# TRAINING_REGIONS_BED=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_training_regions.bed -# HMM_MODEL=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_model.json +# check bedpe for both gaussian, poisson +macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log +macs3 hmmratac -i $ATACSEQBED --hmm-type poisson -n hmmratac_yeast500k_bedpe_poisson -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe_poisson.log -# echo "15.1 hmmratac load training regions from bedfile" -# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log +TRAINING_REGIONS_BED=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_training_regions.bed +HMM_MODEL=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_model.json +TRAINING_REGIONS_BED_POISSON=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_poisson_training_regions.bed +HMM_MODEL_POISSON=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_poisson_model.json + +echo "15.1 hmmratac load training regions from bedfile" +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log +macs3 hmmratac -i $ATACSEQBAM --hmmtype poisson -n hmmratac_yeast500k_poisson_load_training_regions -t ${TRAINING_REGIONS_BED_POISSON} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions_poisson.log # echo "15.2 hmmratac load hmm model file" -# macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log +macs3 hmmratac -i $ATACSEQBAM --hmm-type poisson -n hmmratac_yeast500k_poisson_load_hmm_model --model ${HMM_MODEL_POISSON} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model_poisson.log echo "16. search for errors or warnings in log files" flag=0 diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak new file mode 100644 index 00000000..a877edea --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak @@ -0,0 +1,929 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 +chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 0 +chrI 60100 62170 peak_4 0 . 60900 61820 0 2 570,270 800,1450 0 0 0 +chrI 62620 63520 peak_5 0 . 62630 62760 0 1 130 10 0 0 0 +chrI 67960 69560 peak_6 0 . 68170 69180 0 1 1010 210 0 0 0 +chrI 70980 71590 peak_7 0 . 71080 71510 0 1 430 100 0 0 0 +chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 +chrI 139050 139300 peak_9 0 . 139070 139270 0 1 200 20 0 0 0 +chrI 140550 142040 peak_10 0 . 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/dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_poisson_accessible_regions.gappedPeak @@ -0,0 +1,729 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 45180 45720 peak_2 0 . 45290 45590 0 1 300 110 0 0 0 +chrI 60720 61940 peak_3 0 . 61130 61290 0 1 160 410 0 0 0 +chrI 67960 69790 peak_4 0 . 68170 68960 0 1 790 210 0 0 0 +chrI 70930 71590 peak_5 0 . 71090 71510 0 1 420 160 0 0 0 +chrI 139030 139300 peak_6 0 . 139070 139210 0 1 140 40 0 0 0 +chrI 140570 142240 peak_7 0 . 141550 141950 0 1 400 980 0 0 0 +chrI 165050 165900 peak_8 0 . 165490 165830 0 1 340 440 0 0 0 +chrI 166090 166480 peak_9 0 . 166200 166370 0 1 170 110 0 0 0 +chrI 180850 181500 peak_10 0 . 181040 181310 0 1 270 190 0 0 0 +chrI 182330 182740 peak_11 0 . 182450 182670 0 1 220 120 0 0 0 +chrI 189850 192060 peak_12 0 . 190020 190670 0 1 650 170 0 0 0 +chrII 5290 6510 peak_13 0 . 5520 6370 0 1 850 230 0 0 0 +chrII 36120 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50 0 0 0 +chrIV 591530 592510 peak_107 0 . 591640 592350 0 1 710 110 0 0 0 +chrIV 593230 594240 peak_108 0 . 593420 593580 0 1 160 190 0 0 0 +chrIV 599670 602030 peak_109 0 . 599800 600380 0 1 580 130 0 0 0 +chrIV 635820 636650 peak_110 0 . 636180 636320 0 1 140 360 0 0 0 +chrIV 650400 651150 peak_111 0 . 650710 650940 0 1 230 310 0 0 0 +chrIV 721560 722200 peak_112 0 . 721760 722110 0 1 350 200 0 0 0 +chrIV 768490 770170 peak_113 0 . 768850 769550 0 1 700 360 0 0 0 +chrIV 805950 807510 peak_114 0 . 806210 806640 0 1 430 260 0 0 0 +chrIV 811400 812360 peak_115 0 . 811720 812160 0 1 440 320 0 0 0 +chrIV 835340 836820 peak_116 0 . 835800 836330 0 1 530 460 0 0 0 +chrIV 866580 868100 peak_117 0 . 867640 868020 0 1 380 1060 0 0 0 +chrIV 883200 883930 peak_118 0 . 883510 883670 0 1 160 310 0 0 0 +chrIV 884370 884600 peak_119 0 . 884390 884540 0 1 150 20 0 0 0 +chrIV 893980 894370 peak_120 0 . 894020 894140 0 1 120 40 0 0 0 +chrIV 909230 909830 peak_121 0 . 909500 909680 0 1 180 270 0 0 0 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1202030 0 1 110 250 0 0 0 +chrIV 1206940 1207720 peak_137 0 . 1207070 1207400 0 1 330 130 0 0 0 +chrIV 1242200 1243040 peak_138 0 . 1242310 1243010 0 1 700 110 0 0 0 +chrIV 1251490 1252100 peak_139 0 . 1251760 1251950 0 1 190 270 0 0 0 +chrIV 1266640 1267340 peak_140 0 . 1266950 1267180 0 1 230 310 0 0 0 +chrIV 1278590 1279160 peak_141 0 . 1278760 1278910 0 1 150 170 0 0 0 +chrIV 1300630 1301790 peak_142 0 . 1301070 1301490 0 1 420 440 0 0 0 +chrIV 1345250 1345800 peak_143 0 . 1345390 1345540 0 1 150 140 0 0 0 +chrIV 1355650 1356330 peak_144 0 . 1355680 1355890 0 1 210 30 0 0 0 +chrIV 1359510 1359990 peak_145 0 . 1359530 1359820 0 1 290 20 0 0 0 +chrIV 1362340 1362670 peak_146 0 . 1362430 1362620 0 1 190 90 0 0 0 +chrIV 1385550 1386470 peak_147 0 . 1385760 1386050 0 1 290 210 0 0 0 +chrIV 1401170 1401900 peak_148 0 . 1401380 1401620 0 1 240 210 0 0 0 +chrIV 1420280 1420920 peak_149 0 . 1420440 1420820 0 1 380 160 0 0 0 +chrIV 1428910 1430150 peak_150 0 . 1429090 1429490 0 1 400 180 0 0 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242160 242410 0 1 250 210 0 0 0 +chrV 268410 268740 peak_230 0 . 268440 268570 0 1 130 30 0 0 0 +chrV 270200 270610 peak_231 0 . 270300 270490 0 1 190 100 0 0 0 +chrV 280900 281720 peak_232 0 . 280930 281530 0 1 600 30 0 0 0 +chrV 284430 285090 peak_233 0 . 284510 285050 0 1 540 80 0 0 0 +chrV 305900 306340 peak_234 0 . 305940 306260 0 1 320 40 0 0 0 +chrV 311140 312210 peak_235 0 . 311530 312140 0 1 610 390 0 0 0 +chrV 334680 336610 peak_236 0 . 335410 335980 0 1 570 730 0 0 0 +chrV 342440 343320 peak_237 0 . 342490 342800 0 1 310 50 0 0 0 +chrV 349180 350090 peak_238 0 . 349460 349830 0 1 370 280 0 0 0 +chrV 354850 355340 peak_239 0 . 354940 355240 0 1 300 90 0 0 0 +chrV 361610 362660 peak_240 0 . 362380 362610 0 1 230 770 0 0 0 +chrV 386890 387160 peak_241 0 . 386930 387090 0 1 160 40 0 0 0 +chrV 395890 396690 peak_242 0 . 396330 396640 0 1 310 440 0 0 0 +chrV 400390 401410 peak_243 0 . 400870 401030 0 1 160 480 0 0 0 +chrV 421970 422950 peak_244 0 . 422560 422780 0 1 220 590 0 0 0 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80 0 0 0 +chrVI 269840 270110 peak_276 0 . 269960 270100 0 1 140 120 0 0 0 +chrVII 0 560 peak_277 0 . 10 540 0 1 530 10 0 0 0 +chrVII 23060 23800 peak_278 0 . 23340 23750 0 1 410 280 0 0 0 +chrVII 73840 74540 peak_279 0 . 73870 74500 0 2 250,140 30,520 0 0 0 +chrVII 115310 115920 peak_280 0 . 115410 115820 0 1 410 100 0 0 0 +chrVII 122020 122520 peak_281 0 . 122180 122430 0 1 250 160 0 0 0 +chrVII 148940 149430 peak_282 0 . 149080 149380 0 1 300 140 0 0 0 +chrVII 190840 191540 peak_283 0 . 190920 191040 0 1 120 80 0 0 0 +chrVII 205440 205760 peak_284 0 . 205530 205670 0 1 140 90 0 0 0 +chrVII 270770 272240 peak_285 0 . 271120 272230 0 1 1110 350 0 0 0 +chrVII 276760 277600 peak_286 0 . 277120 277420 0 1 300 360 0 0 0 +chrVII 287150 287530 peak_287 0 . 287300 287510 0 1 210 150 0 0 0 +chrVII 306080 307010 peak_288 0 . 306360 306520 0 1 160 280 0 0 0 +chrVII 319620 320190 peak_289 0 . 319710 319940 0 1 230 90 0 0 0 +chrVII 324490 325100 peak_290 0 . 324750 324880 0 1 130 260 0 0 0 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740,110 130,1380 0 0 0 +chrVII 1025910 1027000 peak_336 0 . 1026010 1026910 0 2 210,180 100,820 0 0 0 +chrVII 1050210 1050630 peak_337 0 . 1050310 1050540 0 1 230 100 0 0 0 +chrVII 1051890 1052500 peak_338 0 . 1052310 1052460 0 1 150 420 0 0 0 +chrVII 1065030 1065360 peak_339 0 . 1065220 1065330 0 1 110 190 0 0 0 +chrVII 1083710 1084910 peak_340 0 . 1083850 1084670 0 1 820 140 0 0 0 +chrVII 1090680 1090910 peak_341 0 . 1090720 1090900 0 1 180 40 0 0 0 +chrVIII 4760 5410 peak_342 0 . 4960 5270 0 1 310 200 0 0 0 +chrVIII 47960 48840 peak_343 0 . 48070 48550 0 1 480 110 0 0 0 +chrVIII 50280 50970 peak_344 0 . 50720 50890 0 1 170 440 0 0 0 +chrVIII 54130 55860 peak_345 0 . 55400 55600 0 1 200 1270 0 0 0 +chrVIII 55900 56290 peak_346 0 . 56070 56240 0 1 170 170 0 0 0 +chrVIII 97740 99090 peak_347 0 . 98210 98700 0 1 490 470 0 0 0 +chrVIII 115790 116260 peak_348 0 . 116000 116150 0 1 150 210 0 0 0 +chrVIII 122010 122720 peak_349 0 . 122180 122660 0 1 480 170 0 0 0 +chrVIII 125920 126430 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190 0 0 0 +chrIV 1345270 1345610 peak_190 0 . 1345370 1345570 0 1 200 100 0 0 0 +chrIV 1355650 1355950 peak_191 0 . 1355660 1355890 0 1 230 10 0 0 0 +chrIV 1362340 1362660 peak_192 0 . 1362430 1362640 0 1 210 90 0 0 0 +chrIV 1385550 1386640 peak_193 0 . 1385690 1386310 0 1 620 140 0 0 0 +chrIV 1401320 1402000 peak_194 0 . 1401350 1401630 0 1 280 30 0 0 0 +chrIV 1410310 1412180 peak_195 0 . 1410480 1411180 0 1 700 170 0 0 0 +chrIV 1419730 1421560 peak_196 0 . 1420410 1420980 0 1 570 680 0 0 0 +chrIV 1429000 1429950 peak_197 0 . 1429080 1429740 0 2 410,150 80,590 0 0 0 +chrIV 1450920 1451160 peak_198 0 . 1450930 1451090 0 1 160 10 0 0 0 +chrIV 1459480 1460210 peak_199 0 . 1459510 1459920 0 1 410 30 0 0 0 +chrIV 1461540 1461900 peak_200 0 . 1461640 1461810 0 1 170 100 0 0 0 +chrIV 1468560 1469700 peak_201 0 . 1468620 1469330 0 1 710 60 0 0 0 +chrIV 1476190 1478230 peak_202 0 . 1476730 1477470 0 1 740 540 0 0 0 +chrIV 1489890 1490900 peak_203 0 . 1489980 1490860 0 1 880 90 0 0 0 +chrIV 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174580 175170 peak_219 0 . 174990 175140 0 1 150 410 0 0 0 +chrIX 197600 197820 peak_220 0 . 197610 197810 0 1 200 10 0 0 0 +chrIX 207230 207860 peak_221 0 . 207420 207700 0 1 280 190 0 0 0 +chrIX 210580 210830 peak_222 0 . 210590 210820 0 1 230 10 0 0 0 +chrIX 232340 232890 peak_223 0 . 232480 232850 0 1 370 140 0 0 0 +chrIX 248500 249870 peak_224 0 . 248720 249540 0 2 170,560 220,480 0 0 0 +chrIX 253850 254930 peak_225 0 . 254170 254790 0 1 620 320 0 0 0 +chrIX 257130 257700 peak_226 0 . 257210 257590 0 1 380 80 0 0 0 +chrIX 258480 258790 peak_227 0 . 258500 258620 0 1 120 20 0 0 0 +chrIX 267820 268450 peak_228 0 . 267950 268270 0 1 320 130 0 0 0 +chrIX 316300 316660 peak_229 0 . 316310 316640 0 1 330 10 0 0 0 +chrIX 324260 324490 peak_230 0 . 324310 324440 0 1 130 50 0 0 0 +chrIX 325660 326210 peak_231 0 . 325710 325870 0 1 160 50 0 0 0 +chrIX 332620 333490 peak_232 0 . 332690 333480 0 1 790 70 0 0 0 +chrIX 381890 383310 peak_233 0 . 382180 382890 0 2 320,330 290,670 0 0 0 +chrIX 383760 384880 peak_234 0 . 384230 384540 0 1 310 470 0 0 0 +chrIX 386010 387060 peak_235 0 . 386550 386930 0 1 380 540 0 0 0 +chrIX 388430 388810 peak_236 0 . 388510 388690 0 1 180 80 0 0 0 +chrM 6090 6240 peak_237 0 . 6100 6230 0 1 130 10 0 0 0 +chrM 17840 18450 peak_238 0 . 17860 18410 0 2 120,390 20,180 0 0 0 +chrM 21250 21410 peak_239 0 . 21270 21390 0 1 120 20 0 0 0 +chrM 21980 22260 peak_240 0 . 22000 22250 0 1 250 20 0 0 0 +chrM 23590 23770 peak_241 0 . 23600 23760 0 1 160 10 0 0 0 +chrM 28810 29100 peak_242 0 . 28820 29090 0 1 270 10 0 0 0 +chrM 31120 31310 peak_243 0 . 31130 31300 0 1 170 10 0 0 0 +chrM 37570 37760 peak_244 0 . 37580 37750 0 1 170 10 0 0 0 +chrM 41520 42020 peak_245 0 . 41530 41990 0 2 180,160 10,310 0 0 0 +chrM 43230 43630 peak_246 0 . 43300 43620 0 1 320 70 0 0 0 +chrM 50680 50820 peak_247 0 . 50690 50810 0 1 120 10 0 0 0 +chrM 53610 53770 peak_248 0 . 53620 53760 0 1 140 10 0 0 0 +chrM 59790 60070 peak_249 0 . 59800 60050 0 1 250 10 0 0 0 +chrM 60780 60960 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peak_266 0 . 174710 174950 0 1 240 150 0 0 0 +chrV 191640 194070 peak_267 0 . 191880 193780 0 2 770,220 240,1920 0 0 0 +chrV 194810 195360 peak_268 0 . 194950 195060 0 1 110 140 0 0 0 +chrV 212080 212310 peak_269 0 . 212090 212230 0 1 140 10 0 0 0 +chrV 221590 221840 peak_270 0 . 221620 221770 0 1 150 30 0 0 0 +chrV 221920 223150 peak_271 0 . 222240 222560 0 1 320 320 0 0 0 +chrV 225430 225720 peak_272 0 . 225440 225690 0 1 250 10 0 0 0 +chrV 241960 242700 peak_273 0 . 242150 242540 0 1 390 190 0 0 0 +chrV 259920 260350 peak_274 0 . 260150 260260 0 1 110 230 0 0 0 +chrV 268390 269130 peak_275 0 . 268420 268570 0 1 150 30 0 0 0 +chrV 270260 270600 peak_276 0 . 270280 270520 0 1 240 20 0 0 0 +chrV 284440 285090 peak_277 0 . 284510 285050 0 1 540 70 0 0 0 +chrV 303430 303840 peak_278 0 . 303600 303750 0 1 150 170 0 0 0 +chrV 305910 306270 peak_279 0 . 305940 306260 0 1 320 30 0 0 0 +chrV 311180 312180 peak_280 0 . 311510 312160 0 1 650 330 0 0 0 +chrV 334590 336790 peak_281 0 . 335420 335980 0 1 560 830 0 0 0 +chrV 342470 343100 peak_282 0 . 342480 342800 0 1 320 10 0 0 0 +chrV 349130 350180 peak_283 0 . 349450 349850 0 1 400 320 0 0 0 +chrV 354850 355320 peak_284 0 . 354860 355240 0 1 380 10 0 0 0 +chrV 362350 362630 peak_285 0 . 362380 362620 0 1 240 30 0 0 0 +chrV 386910 387460 peak_286 0 . 386920 387100 0 1 180 10 0 0 0 +chrV 395640 396680 peak_287 0 . 396310 396660 0 1 350 670 0 0 0 +chrV 399330 401380 peak_288 0 . 400760 401100 0 1 340 1430 0 0 0 +chrV 422470 423170 peak_289 0 . 422550 422800 0 1 250 80 0 0 0 +chrV 434410 434690 peak_290 0 . 434420 434680 0 1 260 10 0 0 0 +chrV 435520 435890 peak_291 0 . 435580 435790 0 1 210 60 0 0 0 +chrV 438780 439770 peak_292 0 . 439290 439400 0 1 110 510 0 0 0 +chrV 441600 442770 peak_293 0 . 441890 442530 0 1 640 290 0 0 0 +chrV 448080 449030 peak_294 0 . 448540 448990 0 1 450 460 0 0 0 +chrV 461150 462850 peak_295 0 . 461530 462470 0 2 740,140 380,1180 0 0 0 +chrV 473210 475830 peak_296 0 . 473500 475070 0 2 310,990 290,870 0 0 0 +chrV 490400 491430 peak_297 0 . 490950 491400 0 1 450 550 0 0 0 +chrV 492250 492490 peak_298 0 . 492280 492430 0 1 150 30 0 0 0 +chrV 497370 498140 peak_299 0 . 497680 497920 0 1 240 310 0 0 0 +chrV 544450 545350 peak_300 0 . 544620 545340 0 1 720 170 0 0 0 +chrV 546550 548050 peak_301 0 . 546620 546980 0 1 360 70 0 0 0 +chrV 551170 551440 peak_302 0 . 551230 551430 0 1 200 60 0 0 0 +chrV 569690 570730 peak_303 0 . 569700 570640 0 1 940 10 0 0 0 +chrVI 4280 5300 peak_304 0 . 4340 5290 0 1 950 60 0 0 0 +chrVI 74640 75670 peak_305 0 . 74710 75000 0 1 290 70 0 0 0 +chrVI 82030 82450 peak_306 0 . 82090 82390 0 1 300 60 0 0 0 +chrVI 90020 90930 peak_307 0 . 90340 90900 0 1 560 320 0 0 0 +chrVI 95130 96030 peak_308 0 . 95180 95930 0 1 750 50 0 0 0 +chrVI 101380 101520 peak_309 0 . 101390 101500 0 1 110 10 0 0 0 +chrVI 106290 106750 peak_310 0 . 106320 106740 0 1 420 30 0 0 0 +chrVI 157850 158080 peak_311 0 . 157880 158070 0 1 190 30 0 0 0 +chrVI 176580 177220 peak_312 0 . 176710 176900 0 1 190 130 0 0 0 +chrVI 183020 185320 peak_313 0 . 183030 184780 0 1 1750 10 0 0 0 +chrVI 191100 191720 peak_314 0 . 191470 191690 0 1 220 370 0 0 0 +chrVI 210280 211740 peak_315 0 . 210660 210820 0 1 160 380 0 0 0 +chrVI 220870 221970 peak_316 0 . 221430 221950 0 1 520 560 0 0 0 +chrVI 224400 226590 peak_317 0 . 224490 226480 0 1 1990 90 0 0 0 +chrVI 226680 227440 peak_318 0 . 226720 226860 0 1 140 40 0 0 0 +chrVII 22850 23770 peak_319 0 . 23350 23750 0 1 400 500 0 0 0 +chrVII 54400 57140 peak_320 0 . 56200 56500 0 1 300 1800 0 0 0 +chrVII 73840 74710 peak_321 0 . 73870 74640 0 2 270,310 30,490 0 0 0 +chrVII 76490 76700 peak_322 0 . 76570 76690 0 1 120 80 0 0 0 +chrVII 80770 82330 peak_323 0 . 81240 81370 0 1 130 470 0 0 0 +chrVII 94910 95680 peak_324 0 . 95310 95530 0 1 220 400 0 0 0 +chrVII 110350 110780 peak_325 0 . 110550 110680 0 1 130 200 0 0 0 +chrVII 115400 115910 peak_326 0 . 115410 115850 0 1 440 10 0 0 0 +chrVII 122040 122520 peak_327 0 . 122180 122430 0 1 250 140 0 0 0 +chrVII 124000 124550 peak_328 0 . 124300 124430 0 1 130 300 0 0 0 +chrVII 148960 149420 peak_329 0 . 149080 149380 0 1 300 120 0 0 0 +chrVII 165740 166440 peak_330 0 . 166020 166190 0 1 170 280 0 0 0 +chrVII 190850 191730 peak_331 0 . 190920 191080 0 1 160 70 0 0 0 +chrVII 196680 197550 peak_332 0 . 197130 197470 0 1 340 450 0 0 0 +chrVII 205450 206030 peak_333 0 . 205530 205670 0 1 140 80 0 0 0 +chrVII 206400 206670 peak_334 0 . 206450 206650 0 1 200 50 0 0 0 +chrVII 254220 255250 peak_335 0 . 254340 254480 0 1 140 120 0 0 0 +chrVII 270220 272250 peak_336 0 . 270880 272230 0 1 1350 660 0 0 0 +chrVII 272440 273590 peak_337 0 . 272720 273180 0 1 460 280 0 0 0 +chrVII 276380 277560 peak_338 0 . 277120 277420 0 1 300 740 0 0 0 +chrVII 287040 287530 peak_339 0 . 287280 287520 0 1 240 240 0 0 0 +chrVII 305970 307060 peak_340 0 . 306350 306620 0 1 270 380 0 0 0 +chrVII 310610 310860 peak_341 0 . 310620 310840 0 1 220 10 0 0 0 +chrVII 319630 320030 peak_342 0 . 319700 319940 0 1 240 70 0 0 0 +chrVII 324630 325050 peak_343 0 . 324730 324880 0 1 150 100 0 0 0 +chrVII 345600 346070 peak_344 0 . 345640 345790 0 1 150 40 0 0 0 +chrVII 370890 371640 peak_345 0 . 371200 371590 0 1 390 310 0 0 0 +chrVII 383400 385190 peak_346 0 . 384270 385040 0 1 770 870 0 0 0 +chrVII 401360 401700 peak_347 0 . 401430 401680 0 1 250 70 0 0 0 +chrVII 405330 406000 peak_348 0 . 405490 405650 0 1 160 160 0 0 0 +chrVII 412810 413580 peak_349 0 . 413250 413490 0 1 240 440 0 0 0 +chrVII 423010 423490 peak_350 0 . 423100 423320 0 1 220 90 0 0 0 +chrVII 428120 428400 peak_351 0 . 428140 428390 0 1 250 20 0 0 0 +chrVII 438310 438810 peak_352 0 . 438500 438770 0 1 270 190 0 0 0 +chrVII 483070 484070 peak_353 0 . 483270 483890 0 1 620 200 0 0 0 +chrVII 515780 516550 peak_354 0 . 516150 516520 0 1 370 370 0 0 0 +chrVII 527710 529080 peak_355 0 . 528320 528870 0 2 140,240 610,920 0 0 0 +chrVII 536030 537000 peak_356 0 . 536120 536480 0 1 360 90 0 0 0 +chrVII 546470 547870 peak_357 0 . 546950 547670 0 1 720 480 0 0 0 +chrVII 555450 555960 peak_358 0 . 555480 555670 0 1 190 30 0 0 0 +chrVII 559920 560470 peak_359 0 . 560130 560450 0 1 320 210 0 0 0 +chrVII 566770 567490 peak_360 0 . 567060 567310 0 1 250 290 0 0 0 +chrVII 576430 576910 peak_361 0 . 576610 576800 0 1 190 180 0 0 0 +chrVII 581610 581920 peak_362 0 . 581790 581910 0 1 120 180 0 0 0 +chrVII 592090 593240 peak_363 0 . 592510 593110 0 1 600 420 0 0 0 +chrVII 598850 599200 peak_364 0 . 598910 599180 0 1 270 60 0 0 0 +chrVII 609200 610360 peak_365 0 . 609750 610210 0 1 460 550 0 0 0 +chrVII 625210 625790 peak_366 0 . 625240 625600 0 1 360 30 0 0 0 +chrVII 640660 641510 peak_367 0 . 640850 641340 0 1 490 190 0 0 0 +chrVII 650380 651130 peak_368 0 . 650760 650890 0 1 130 380 0 0 0 +chrVII 653880 654640 peak_369 0 . 654130 654270 0 1 140 250 0 0 0 +chrVII 661700 661990 peak_370 0 . 661770 661980 0 1 210 70 0 0 0 +chrVII 700570 701110 peak_371 0 . 700670 701030 0 1 360 100 0 0 0 +chrVII 702690 703270 peak_372 0 . 702900 703030 0 1 130 210 0 0 0 +chrVII 706810 707310 peak_373 0 . 707080 707240 0 1 160 270 0 0 0 +chrVII 726640 726920 peak_374 0 . 726650 726870 0 1 220 10 0 0 0 +chrVII 731690 733080 peak_375 0 . 731980 732110 0 1 130 290 0 0 0 +chrVII 753650 754550 peak_376 0 . 753970 754140 0 1 170 320 0 0 0 +chrVII 756080 757400 peak_377 0 . 756560 756810 0 1 250 480 0 0 0 +chrVII 765940 766830 peak_378 0 . 766000 766600 0 1 600 60 0 0 0 +chrVII 771800 772340 peak_379 0 . 772000 772280 0 1 280 200 0 0 0 +chrVII 774270 774850 peak_380 0 . 774370 774830 0 1 460 100 0 0 0 +chrVII 785350 787170 peak_381 0 . 785500 787140 0 2 730,110 150,1680 0 0 0 +chrVII 788060 788810 peak_382 0 . 788280 788670 0 1 390 220 0 0 0 +chrVII 809530 810740 peak_383 0 . 809660 810140 0 1 480 130 0 0 0 +chrVII 810880 811390 peak_384 0 . 810920 811050 0 1 130 40 0 0 0 +chrVII 811700 812470 peak_385 0 . 812010 812140 0 1 130 310 0 0 0 +chrVII 821450 823020 peak_386 0 . 822580 822860 0 1 280 1130 0 0 0 +chrVII 845570 845750 peak_387 0 . 845580 845740 0 1 160 10 0 0 0 +chrVII 856410 858130 peak_388 0 . 856460 857720 0 2 820,310 50,1000 0 0 0 +chrVII 859070 859760 peak_389 0 . 859460 859710 0 1 250 390 0 0 0 +chrVII 882840 883820 peak_390 0 . 882890 883690 0 1 800 50 0 0 0 +chrVII 883920 884410 peak_391 0 . 883970 884380 0 1 410 50 0 0 0 +chrVII 904940 905820 peak_392 0 . 905010 905810 0 1 800 70 0 0 0 +chrVII 912960 915600 peak_393 0 . 914760 915150 0 2 110,190 1800,2000 0 0 0 +chrVII 920120 920640 peak_394 0 . 920130 920540 0 1 410 10 0 0 0 +chrVII 974260 974770 peak_395 0 . 974520 974740 0 1 220 260 0 0 0 +chrVII 976750 977190 peak_396 0 . 976850 977100 0 1 250 100 0 0 0 +chrVII 986840 987830 peak_397 0 . 986860 987680 0 2 110,670 20,170 0 0 0 +chrVII 993790 994650 peak_398 0 . 993980 994280 0 1 300 190 0 0 0 +chrVII 1000000 1002190 peak_399 0 . 1000020 1001420 0 2 750,460 20,960 0 0 0 +chrVII 1025950 1027480 peak_400 0 . 1025990 1027210 0 2 220,510 40,750 0 0 0 +chrVII 1050230 1050620 peak_401 0 . 1050320 1050540 0 1 220 90 0 0 0 +chrVII 1052220 1052480 peak_402 0 . 1052310 1052470 0 1 160 90 0 0 0 +chrVII 1083720 1084830 peak_403 0 . 1083730 1084680 0 1 950 10 0 0 0 +chrVII 1086460 1087950 peak_404 0 . 1087030 1087500 0 1 470 570 0 0 0 +chrVIII 4610 5410 peak_405 0 . 4870 5400 0 1 530 260 0 0 0 +chrVIII 48020 48600 peak_406 0 . 48070 48560 0 1 490 50 0 0 0 +chrVIII 50530 50940 peak_407 0 . 50720 50900 0 1 180 190 0 0 0 +chrVIII 54700 55800 peak_408 0 . 55270 55740 0 1 470 570 0 0 0 +chrVIII 55900 56320 peak_409 0 . 56070 56240 0 1 170 170 0 0 0 +chrVIII 62630 62860 peak_410 0 . 62650 62780 0 1 130 20 0 0 0 +chrVIII 97740 99020 peak_411 0 . 98210 99000 0 1 790 470 0 0 0 +chrVIII 115820 116410 peak_412 0 . 116000 116160 0 1 160 180 0 0 0 +chrVIII 122040 122710 peak_413 0 . 122180 122660 0 1 480 140 0 0 0 +chrVIII 125920 126410 peak_414 0 . 126000 126360 0 1 360 80 0 0 0 +chrVIII 142980 144370 peak_415 0 . 143490 143830 0 1 340 510 0 0 0 +chrVIII 148790 149020 peak_416 0 . 148820 149000 0 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a/test/standard_results_hmmratac/hmmratac_yeast500k_load_training_regions_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_load_training_regions_accessible_regions.gappedPeak new file mode 100644 index 00000000..a877edea --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_load_training_regions_accessible_regions.gappedPeak @@ -0,0 +1,929 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 31650 34930 peak_2 0 . 32850 33100 0 1 250 1200 0 0 0 +chrI 45220 45620 peak_3 0 . 45290 45610 0 1 320 70 0 0 0 +chrI 60100 62170 peak_4 0 . 60900 61820 0 2 570,270 800,1450 0 0 0 +chrI 62620 63520 peak_5 0 . 62630 62760 0 1 130 10 0 0 0 +chrI 67960 69560 peak_6 0 . 68170 69180 0 1 1010 210 0 0 0 +chrI 70980 71590 peak_7 0 . 71080 71510 0 1 430 100 0 0 0 +chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 +chrI 139050 139300 peak_9 0 . 139070 139270 0 1 200 20 0 0 0 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new file mode 100644 index 00000000..38810eb7 --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_hmm_model_accessible_regions.gappedPeak @@ -0,0 +1,729 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 45180 45720 peak_2 0 . 45290 45590 0 1 300 110 0 0 0 +chrI 60720 61940 peak_3 0 . 61130 61290 0 1 160 410 0 0 0 +chrI 67960 69790 peak_4 0 . 68170 68960 0 1 790 210 0 0 0 +chrI 70930 71590 peak_5 0 . 71090 71510 0 1 420 160 0 0 0 +chrI 139030 139300 peak_6 0 . 139070 139210 0 1 140 40 0 0 0 +chrI 140570 142240 peak_7 0 . 141550 141950 0 1 400 980 0 0 0 +chrI 165050 165900 peak_8 0 . 165490 165830 0 1 340 440 0 0 0 +chrI 166090 166480 peak_9 0 . 166200 166370 0 1 170 110 0 0 0 +chrI 180850 181500 peak_10 0 . 181040 181310 0 1 270 190 0 0 0 +chrI 182330 182740 peak_11 0 . 182450 182670 0 1 220 120 0 0 0 +chrI 189850 192060 peak_12 0 . 190020 190670 0 1 650 170 0 0 0 +chrII 5290 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860250 860740 0 1 490 10 0 0 0 +chrXVI 880230 880530 peak_726 0 . 880270 880430 0 1 160 40 0 0 0 +chrXVI 927510 928500 peak_727 0 . 927870 928180 0 1 310 360 0 0 0 +chrXVI 942450 942850 peak_728 0 . 942570 942730 0 1 160 120 0 0 0 diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_training_regions_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_training_regions_accessible_regions.gappedPeak new file mode 100644 index 00000000..38810eb7 --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_poisson_load_training_regions_accessible_regions.gappedPeak @@ -0,0 +1,729 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 45180 45720 peak_2 0 . 45290 45590 0 1 300 110 0 0 0 +chrI 60720 61940 peak_3 0 . 61130 61290 0 1 160 410 0 0 0 +chrI 67960 69790 peak_4 0 . 68170 68960 0 1 790 210 0 0 0 +chrI 70930 71590 peak_5 0 . 71090 71510 0 1 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peak_703 0 . 482100 482350 0 1 250 240 0 0 0 +chrXVI 502370 503250 peak_704 0 . 502480 503080 0 2 150,350 110,360 0 0 0 +chrXVI 519980 520610 peak_705 0 . 520260 520510 0 1 250 280 0 0 0 +chrXVI 526720 527480 peak_706 0 . 526980 527180 0 1 200 260 0 0 0 +chrXVI 572270 572560 peak_707 0 . 572330 572450 0 1 120 60 0 0 0 +chrXVI 585860 586590 peak_708 0 . 586100 586260 0 1 160 240 0 0 0 +chrXVI 641180 641910 peak_709 0 . 641250 641790 0 1 540 70 0 0 0 +chrXVI 644340 645800 peak_710 0 . 645050 645690 0 1 640 710 0 0 0 +chrXVI 678330 679440 peak_711 0 . 678610 679300 0 1 690 280 0 0 0 +chrXVI 694640 695540 peak_712 0 . 694980 695280 0 1 300 340 0 0 0 +chrXVI 700050 700370 peak_713 0 . 700080 700320 0 1 240 30 0 0 0 +chrXVI 731850 732250 peak_714 0 . 731890 732210 0 1 320 40 0 0 0 +chrXVI 769130 769450 peak_715 0 . 769180 769370 0 1 190 50 0 0 0 +chrXVI 770520 771080 peak_716 0 . 770880 771060 0 1 180 360 0 0 0 +chrXVI 794270 795060 peak_717 0 . 794620 794880 0 1 260 350 0 0 0 +chrXVI 809540 810340 peak_718 0 . 809830 810210 0 1 380 290 0 0 0 +chrXVI 821970 822540 peak_719 0 . 822090 822380 0 1 290 120 0 0 0 +chrXVI 828650 829580 peak_720 0 . 828830 829520 0 1 690 180 0 0 0 +chrXVI 840140 841060 peak_721 0 . 840280 840830 0 2 310,120 140,570 0 0 0 +chrXVI 844730 845540 peak_722 0 . 844900 845190 0 1 290 170 0 0 0 +chrXVI 855400 856300 peak_723 0 . 855770 856190 0 1 420 370 0 0 0 +chrXVI 856780 857080 peak_724 0 . 856820 857000 0 1 180 40 0 0 0 +chrXVI 860240 860900 peak_725 0 . 860250 860740 0 1 490 10 0 0 0 +chrXVI 880230 880530 peak_726 0 . 880270 880430 0 1 160 40 0 0 0 +chrXVI 927510 928500 peak_727 0 . 927870 928180 0 1 310 360 0 0 0 +chrXVI 942450 942850 peak_728 0 . 942570 942730 0 1 160 120 0 0 0 From d00970e9fcc5dba9d46acd2eb4daa94e73058ae7 Mon Sep 17 00:00:00 2001 From: philippadoherty Date: Fri, 15 Mar 2024 15:52:36 -0400 Subject: [PATCH 7/7] retry hmmratac cmdline tests --- test/cmdlinetest | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/test/cmdlinetest b/test/cmdlinetest index e0ba4abc..568f954f 100755 --- a/test/cmdlinetest +++ b/test/cmdlinetest @@ -231,7 +231,7 @@ HMM_MODEL_POISSON=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_poisson_mo echo "15.1 hmmratac load training regions from bedfile" macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log -macs3 hmmratac -i $ATACSEQBAM --hmmtype poisson -n hmmratac_yeast500k_poisson_load_training_regions -t ${TRAINING_REGIONS_BED_POISSON} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions_poisson.log +macs3 hmmratac -i $ATACSEQBAM --hmm-type poisson -n hmmratac_yeast500k_poisson_load_training_regions -t ${TRAINING_REGIONS_BED_POISSON} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions_poisson.log # echo "15.2 hmmratac load hmm model file" macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log