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problem when run get-reads-alt-unmap.sh #47

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Huangpq2019 opened this issue Jul 9, 2019 · 0 comments
Open

problem when run get-reads-alt-unmap.sh #47

Huangpq2019 opened this issue Jul 9, 2019 · 0 comments

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@Huangpq2019
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When I run get-reads-alt-unmap.sh in.bam out.bam to process my data, there is an error message:

*****WARNING: Query ERR091574.99978736 is marked as paired, but its mate does not occur next to it in your BAM file. Skipping.
...
*****WARNING: Query ERR091574.9999745 is marked as paired, but its mate does not occur next to it in your BAM file. Skipping.

sambamba 0.7.0
by Artem Tarasov and Pjotr Prins (C) 2012-2019
LDC 1.13.0 / DMD v2.083.1 / LLVM7.0.1 / bootstrap LDC - the LLVM D compiler (0.17.6)

[bam_header_read] EOF marker is absent. The input is probably truncated.
[bwt_restore_sa] SA-BWT inconsistency: primary is not the same. Abort!
[bam_header_read] invalid BAM binary header (this is not a BAM file).
[main_samview] fail to read the header from "-".
sambamba-sort: not enough data in stream
rm: cannot remove 'out.bam.full.bam': No such file or directory
rm: cannot remove 'out.bam.full.bam.bai': No such file or directory

How can I solve this problem?

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