ethoscope tracking database columns "is inferred" and "has interacted"

[rjs360]

Hi all,
Finished my first few ethoscope tracking experiments with sleep deprivator units (https://giorgiogilestro.notion.site/The-new-Modular-SD-Device-05bbe90b6ee04b8aa439165f69d62de8) today (yay!), and notice two columns in the .db files (reading them in "DB Broswer for SQLite" currently) named "is inferred" and "has interacted" which either take "0" or "1" values. Can someone tell me what these two columns indicate exactly? Do "has interacted" of "1" refer to sleep deprivator motor action? Thanks for any shared insight - Ruud

[ggilestro]

Congrats on getting everything to work!

has_interacted will tell you if the fly has triggered an event following the programmed feedback loop settings. In other words, if the motor has rotated after the detected time of immobility (or any other pre-programmed trigger). I can be 0, 1 or 2.

0 -> no interaction
1 -> full interaction 
2 -> interaction detected, but action not triggered because the user set a probability value < 1.0

A probability value of 0 means all 1s are going to be converted to 2s and the motor will never turn.

inferred refers to the position and status of the fly. It means tracking was lost for that frame so the position of the fly is assumed based on previous information.

N.B. I have started the discussion section in the repo a converted this issue to a discussion.

[rjs360]

Great thanks Giorgio!

[Ivanad29]

Dear Gilestro we have recently biuld up ethoscopes based on your development and we have been analyzing data with the python in collab scripts and suddenly the function plot_overtime stop to run it seems objets of the type behavpy do not support this function anymore. Any ideas?

AttributeError Traceback (most recent call last)
in <cell line: 0>()
1 # Crear el gráfico
----> 2 fig = df_48hr.xmv('sex', 'F').plot_overtime(variable='asleep', wrapped=False, facet_col='exp_group', facet_arg = [3, 2, 1], facet_labels = ['CONTROL Elav WHITE', 'CONTROL Trip WHITE', 'EXPERIMENTAL ELAV Trip'], avg_window=180, title='FEMALES')
3
4 # Actualizar diseño
5 fig.update_layout(autosize=False, width=1500, height=500)

/usr/local/lib/python3.11/dist-packages/pandas/core/generic.py in getattr(self, name)
6297 ):
6298 return self[name]
-> 6299 return object.getattribute(self, name)
6300
6301 @Final

AttributeError: 'behavpy' object has no attribute 'plot_overtime'

I tried changing the plot function but the thing is the ones from seaborn or plotly crashes the arguments of sex, and groups since the columns ar not exactly part of the main dataframe

Thanks for your time and help,
Ivana

[ggilestro]

Hi @Ivanad29 - can you please raise this issue in the ethoscopy repo? https://github.com/gilestrolab/ethoscopy/issues
Thanks

[Ivanad29]

Sure, I 've just created a new issue. Thanks