diff --git a/lib/gradient/elixir_checker.ex b/lib/gradient/elixir_checker.ex
index 2b54b424..0cf737c0 100644
--- a/lib/gradient/elixir_checker.ex
+++ b/lib/gradient/elixir_checker.ex
@@ -6,10 +6,14 @@ defmodule Gradient.ElixirChecker do
   - {`ex_check`, boolean()}: whether to use checks specific only to Elixir.
   """
 
-  @spec check([:erl_parse.abstract_form()], keyword()) :: [{:file.filename(), any()}]
+  @type env() :: Gradient.env()
+
+  @type opts :: [env: env(), ex_check: boolean()]
+
+  @spec check([:erl_parse.abstract_form()], opts()) :: [{:file.filename(), any()}]
   def check(forms, opts) do
     if Keyword.get(opts, :ex_check, true) do
-      check_spec(forms)
+      check_spec(forms, opts[:env])
     else
       []
     end
@@ -46,13 +50,16 @@ defmodule Gradient.ElixirChecker do
     end
     ```
   """
-  @spec check_spec([:erl_parse.abstract_form()]) :: [{:file.filename(), any()}]
-  def check_spec([{:attribute, _, :file, {file, _}} | forms]) do
+  @spec check_spec([:erl_parse.abstract_form()], map()) :: [{:file.filename(), any()}]
+  def check_spec([{:attribute, _, :file, {file, _}} | forms], env) do
+    %{tokens_present: tokens_present, macro_lines: macro_lines} = env
+
     forms
-    |> Stream.filter(&is_fun_or_spec?/1)
+    |> Stream.filter(&is_fun_or_spec?(&1, macro_lines))
     |> Stream.map(&simplify_form/1)
     |> Stream.concat()
     |> Stream.filter(&is_not_generated?/1)
+    |> remove_injected_forms(not tokens_present)
     |> Enum.sort(&(elem(&1, 2) < elem(&2, 2)))
     |> Enum.reduce({nil, []}, fn
       {:fun, {n, :def}, _}, {{:spec, {sn, _}, _}, _} = acc when n == sn ->
@@ -81,12 +88,13 @@ defmodule Gradient.ElixirChecker do
     not (String.starts_with?(name_str, "__") and String.ends_with?(name_str, "__"))
   end
 
-  def is_fun_or_spec?({:attribute, _, :spec, _}), do: true
-  def is_fun_or_spec?({:function, _, _, _, _}), do: true
-  def is_fun_or_spec?(_), do: false
+  # The forms injected by `__using__` macro inherit the line from `use` keyword.
+  def is_fun_or_spec?({:attribute, anno, :spec, _}, ml), do: :erl_anno.line(anno) not in ml
+  def is_fun_or_spec?({:function, anno, _, _, _}, ml), do: :erl_anno.line(anno) not in ml
+  def is_fun_or_spec?(_, _), do: false
 
-  @spec simplify_form(:erl_parse.abstract_form()) ::
-          Enumerable.t({:spec | :fun, {atom(), integer()}, :erl_anno.anno()})
+  # Returned type Enumerable.t({:spec | :fun, {atom(), integer()}, :erl_anno.anno()})
+  @spec simplify_form(:erl_parse.abstract_form()) :: Enumerable.t()
   def simplify_form({:attribute, _, :spec, {{name, arity}, types}}) do
     Stream.map(types, &{:spec, {name, arity}, elem(&1, 1)})
   end
@@ -111,4 +119,15 @@ defmodule Gradient.ElixirChecker do
       _ -> false
     end)
   end
+
+  # When tokens were not present to detect macro_lines, the forms without unique
+  # lines can be removed.
+  def remove_injected_forms(forms, true) do
+    forms
+    |> Enum.group_by(fn {_, _, line} -> line end)
+    |> Enum.filter(fn {_, fs2} -> length(fs2) == 1 end)
+    |> Enum.flat_map(fn {_, fs2} -> fs2 end)
+  end
+
+  def remove_injected_forms(forms, false), do: forms
 end
diff --git a/test/examples/spec_in_macro.ex b/test/examples/spec_in_macro.ex
new file mode 100644
index 00000000..a29a8ced
--- /dev/null
+++ b/test/examples/spec_in_macro.ex
@@ -0,0 +1,21 @@
+defmodule NewMod do
+  defmacro __using__(_) do
+    quote do
+      @spec new(attrs :: map()) :: atom()
+      def new(_attrs), do: :ok
+
+      @spec a(attrs :: map()) :: atom()
+      def a(_attrs), do: :ok
+
+      @spec b(attrs :: map()) :: atom()
+      def b(_attrs), do: :ok
+    end
+  end
+end
+
+defmodule SpecInMacro do
+  use NewMod
+
+  @spec c(attrs :: map()) :: atom()
+  def c(_attrs), do: :ok
+end
diff --git a/test/gradient/elixir_checker_test.exs b/test/gradient/elixir_checker_test.exs
index e85d9665..b60b576a 100644
--- a/test/gradient/elixir_checker_test.exs
+++ b/test/gradient/elixir_checker_test.exs
@@ -9,27 +9,26 @@ defmodule Gradient.ElixirCheckerTest do
   test "checker options" do
     ast = load("Elixir.SpecWrongName.beam")
 
-    assert [] = ElixirChecker.check(ast, ex_check: false)
-    assert [] != ElixirChecker.check(ast, ex_check: true)
+    assert [] = ElixirChecker.check(ast, env([], ex_check: false))
+    assert [] != ElixirChecker.check(ast, env([], ex_check: true))
   end
 
   test "all specs are correct" do
     ast = load("Elixir.CorrectSpec.beam")
 
-    assert [] = ElixirChecker.check(ast, ex_check: true)
+    assert [] = ElixirChecker.check(ast, env())
   end
 
   test "specs over default args are correct" do
     ast = load("Elixir.SpecDefaultArgs.beam")
 
-    assert [] = ElixirChecker.check(ast, ex_check: true)
+    assert [] = ElixirChecker.check(ast, env())
   end
 
   test "spec arity doesn't match the function arity" do
     ast = load("Elixir.SpecWrongArgsArity.beam")
 
-    assert [{_, {:spec_error, :wrong_spec_name, 2, :foo, 3}}] =
-             ElixirChecker.check(ast, ex_check: true)
+    assert [{_, {:spec_error, :wrong_spec_name, 2, :foo, 3}}] = ElixirChecker.check(ast, env())
   end
 
   test "spec name doesn't match the function name" do
@@ -38,7 +37,7 @@ defmodule Gradient.ElixirCheckerTest do
     assert [
              {_, {:spec_error, :wrong_spec_name, 5, :convert, 1}},
              {_, {:spec_error, :wrong_spec_name, 11, :last_two, 1}}
-           ] = ElixirChecker.check(ast, [])
+           ] = ElixirChecker.check(ast, env())
   end
 
   test "mixing specs names is not allowed" do
@@ -47,6 +46,22 @@ defmodule Gradient.ElixirCheckerTest do
     assert [
              {_, {:spec_error, :mixed_specs, 3, :encode, 1}},
              {_, {:spec_error, :wrong_spec_name, 3, :encode, 1}}
-           ] = ElixirChecker.check(ast, [])
+           ] = ElixirChecker.check(ast, env())
+  end
+
+  test "spec defined in a __using__ macro with tokens" do
+    {tokens, ast} = load("Elixir.SpecInMacro.beam", "spec_in_macro.ex")
+
+    assert [] = ElixirChecker.check(ast, env(tokens))
+  end
+
+  test "spec defined in a __using__ macro without tokens" do
+    ast = load("Elixir.SpecInMacro.beam")
+
+    assert [] = ElixirChecker.check(ast, env())
+  end
+
+  defp env(tokens \\ [], opts \\ []) do
+    [{:env, Gradient.build_env(tokens)} | opts]
   end
 end