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Hic mode finishes without outputing phased contigs #88

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dabitz opened this issue Mar 18, 2021 · 1 comment
Open

Hic mode finishes without outputing phased contigs #88

dabitz opened this issue Mar 18, 2021 · 1 comment

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@dabitz
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dabitz commented Mar 18, 2021

I ran hifiasm with hic mode successfully for another species, but for this one know it failing to output contigs. It finishes the run without any error message at this step "Hi-C linkages have been counted"

[M::ha_analyze_count] left: none
[M::ha_analyze_count] right: count[64] = 277273
[M::ha_pt_gen] peak_hom: 64; peak_het: 32
[M::ha_pt_gen::235095.11134.35] ==> indexed 1826363481 positions
[M::ha_assemble::242771.706
[email protected]] ==> found overlaps for the final round
[M::ha_print_ovlp_stat] # overlaps: 597384192
[M::ha_print_ovlp_stat] # strong overlaps: 382304195
[M::ha_print_ovlp_stat] # weak overlaps: 215079997
[M::ha_print_ovlp_stat] # exact overlaps: 580046562
[M::ha_print_ovlp_stat] # inexact overlaps: 17337630
[M::ha_print_ovlp_stat] # overlaps without large indels: 596554155
[M::ha_print_ovlp_stat] # reverse overlaps: 175924300
Writing reads to disk...
Reads has been written.
Writing ma_hit_ts to disk...
ma_hit_ts has been written.
Writing ma_hit_ts to disk...
ma_hit_ts has been written.
bin files have been written.
[M::purge_dups] purge duplication coverage threshold: 80
[M::purge_dups] purge duplication coverage threshold: 80
[M::adjust_utg_by_primary] primary contig coverage range: [51, infinity]
[M::purge_dups] purge duplication coverage threshold: 80
[M::purge_dups] purge duplication coverage threshold: 80
[M::purge_dups] purge duplication coverage threshold: 80
[M::purge_dups] purge duplication coverage threshold: 80
[M::purge_dups] purge duplication coverage threshold: 80
[M::adjust_utg_by_primary] primary contig coverage range: [51, infinity]
[M::purge_dups] purge duplication coverage threshold: 80
[M::build_unitig_index::141.301] ==> Counting
[M::build_unitig_index::33.765] ==> Memory allocating
[M::build_unitig_index::168.774] ==> Filling pos
[M::build_unitig_index::0.761] ==> Sorting pos
[M::build_unitig_index::344.604] ==> HiC index has been built
[M::write_hc_pt_index] Index has been written.
[M::dedup_hits::13.300] ==> Dedup
[M::all_pair_shortest_path::9.368]
[M::fill_utg_distance_multi::30.110]
[M::collect_hc_links::57.081] ==> Hi-C linkages have been counted

@chhylp123
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=.= Current Hi-C module in hifiasm is not stable, so I'm not sure...Please wait me a few days to release a new version. I guess it will solve most issues.

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