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This gives me ONT reads in fastq format using the PBSim default simulated model. The reads fastq file looks normal.
Then running Hifiasm don't like this:
./hifiasm --ont -o asm -t32 ont_reads.fastq.gz
I am getting the following error message:
the adapterLen of index is: 32766, but the adapterLen set by user is: 0
When running it multiple times on the same dataset the number '32766' changes slightly.
I would like to understand what this error message wants to tell me and what is the problem exactly with the reads?
Since it says something about adapters I also tried chopping adapters from the reads with PoreChop, but it didn't help.
The text was updated successfully, but these errors were encountered:
Hello,
I encounter an issue using Hifiasm ONT. I use synthetic reads sampled from PBSim3 like this:
pbsim --strategy wgs --method qshmm --qshmm data/QSHMM-ONT.model --depth {depth} --genome {ref_path}
This gives me ONT reads in fastq format using the PBSim default simulated model. The reads fastq file looks normal.
Then running Hifiasm don't like this:
./hifiasm --ont -o asm -t32 ont_reads.fastq.gz
I am getting the following error message:
the adapterLen of index is: 32766, but the adapterLen set by user is: 0
When running it multiple times on the same dataset the number '32766' changes slightly.
I would like to understand what this error message wants to tell me and what is the problem exactly with the reads?
Since it says something about adapters I also tried chopping adapters from the reads with PoreChop, but it didn't help.
The text was updated successfully, but these errors were encountered: