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README.md

BioTools

MCP server

General transcriptional regulation dataset and toolkit: MCP server

Simple Cases

Region Annotation

Check TP53 gene coverage on enhancers
Check ESR1, GATA3, FOXA1 gene coverage on related enhancers and SNPs

Expression Analysis

Check TP53 gene expression in TCGA breast cancer

Complex Cases

Case1:

- Upload: /tmp/SRR9091032_1.fastq.gz (Experimental group)
- Upload: /tmp/SRR9091033_1.fastq.gz (Control group)
I have provided 2 single-end sequencing files (hg38), please perform the following analysis:
1. Super enhancer identification and analysis
2. Find core transcriptional regulatory circuitries
- Save path: /tmp/KYSE200_hg38

Case2:

- Upload: /tmp/top200_cardiomyocyte_development.csv
1. Identify key transcription factors
2. Check expression of key transcription factors in normal and cancer tissues
3. Select key cardiac transcription factors and analyze risk SNPs, eQTLs and super enhancers in their binding regions
4. Find target genes of key transcription factors and plot line charts by score
5. Build correlation network of transcription factors and target genes in tissues
- Save path: /tmp/cardiomyocyte