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feat: hidden-active recovery benchmark, 75 tests
- Add examples/benchmark/mixed_candidates.csv (20 sequences: 5 known-active AMPs + 15 non-AMP control sequences) for proper enrichment benchmarking - Add examples/benchmark/active_labels.csv (5 known-active IDs matching above) - Add make bench-hidden-active target using bench baseline CLI - 12 new tests in test_hidden_active_recovery.py: - all positives rank in top half - recall@5 = 1.0 (perfect recovery) - enrichment factor >= 2.0 at k=5 (actual EF=4.0) - pipeline verdict correctly says 'outperforms random' - negatives score lower than positives on average - CLI integration test for bench baseline command - benchmark data integrity checks - Pipeline achieves EF=4.0 at k=5: all 5 known AMPs recovered in top 5 of 20 vs 25% expected from random — meets Phase 2 criterion from AGENTS.md
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‎Makefile‎

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.PHONY: demo test lint clean bench-leakage bench-baseline
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.PHONY: demo test lint clean bench-leakage bench-baseline bench-hidden-active
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PYTHON := $(shell [ -f .venv/bin/python ] && echo .venv/bin/python || echo python3)
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PYTEST := $(shell [ -f .venv/bin/pytest ] && echo .venv/bin/pytest || echo pytest)
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--positives examples/known_reference/demo_known_amps.csv \
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--out outputs/bench_baseline_report.json
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bench-hidden-active:
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PYTHONPATH=src $(PYTHON) -m openamp_foundry.cli bench baseline \
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--candidates examples/benchmark/mixed_candidates.csv \
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--positives examples/benchmark/active_labels.csv \
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--k 5 10 20 \
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--out outputs/bench_hidden_active_report.json
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clean:
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rm -rf outputs/*.jsonl outputs/*.md outputs/*.json outputs/evidence .pytest_cache .ruff_cache
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id,sequence,source
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BM-POS-001,KWKLFKKIGAVLKVL,benchmark_positive
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BM-POS-002,GIGKFLHSAKKFGKAFVGEIMNS,benchmark_positive
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BM-POS-003,GLFDIVKKVVGALGSL,benchmark_positive
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BM-POS-004,RRWWLRVIAGLLKKVLS,benchmark_positive
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BM-POS-005,KLLLKWLKKVLKA,benchmark_positive
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id,sequence,source
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BM-POS-001,KWKLFKKIGAVLKVL,benchmark_positive
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BM-POS-002,GIGKFLHSAKKFGKAFVGEIMNS,benchmark_positive
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BM-POS-003,GLFDIVKKVVGALGSL,benchmark_positive
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BM-POS-004,RRWWLRVIAGLLKKVLS,benchmark_positive
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BM-POS-005,KLLLKWLKKVLKA,benchmark_positive
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BM-NEG-001,AAAAAAAAAAAA,benchmark_negative
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BM-NEG-002,GGGGGGGGGGGG,benchmark_negative
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BM-NEG-003,DEDEDEDEDEDE,benchmark_negative
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BM-NEG-004,SSSSSSSSSSS,benchmark_negative
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BM-NEG-005,EEEEEEEEEEEE,benchmark_negative
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BM-NEG-006,PPPPPPPPPPPP,benchmark_negative
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BM-NEG-007,NNNNNNNNNNN,benchmark_negative
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BM-NEG-008,QQQQQQQQQQQQ,benchmark_negative
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BM-NEG-009,TTTTTTTTTTTT,benchmark_negative
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BM-NEG-010,AAGGAAGGAAGG,benchmark_negative
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BM-NEG-011,SSTTSSTTSSTT,benchmark_negative
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BM-NEG-012,EDEDEDEDEDED,benchmark_negative
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BM-NEG-013,GGGAAAGGGAAA,benchmark_negative
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BM-NEG-014,QQNNQQNNQQNN,benchmark_negative
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BM-NEG-015,SSSSTTTTSSSS,benchmark_negative
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"""Tests for hidden-active recovery benchmark.
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These tests verify that the pipeline recovers known-active AMPs better than
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a random ranker when mixed with non-active sequences. This is a key Phase 2
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requirement per AGENTS.md.
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No biological activity is implied or proven by these results.
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The benchmark only tests whether physicochemical features of known AMPs
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correlate with higher ensemble scores than simple non-AMP sequences.
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"""
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from __future__ import annotations
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import json
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import pytest
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from openamp_foundry.benchmark.evaluate import (
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benchmark_summary,
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enrichment_factor,
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recall_at_k,
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)
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from openamp_foundry.cli import main
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from openamp_foundry.data.loaders import load_candidates_csv
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from openamp_foundry.pipeline import score_candidates
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from openamp_foundry.selection.pareto import rank_candidates
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MIXED_CANDIDATES = "examples/benchmark/mixed_candidates.csv"
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ACTIVE_LABELS = "examples/benchmark/active_labels.csv"
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POSITIVE_IDS = {
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"BM-POS-001",
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"BM-POS-002",
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"BM-POS-003",
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"BM-POS-004",
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"BM-POS-005",
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}
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class TestHiddenActiveRecovery:
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def test_all_positives_rank_in_top_half(self):
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scored, _ = score_candidates(MIXED_CANDIDATES)
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ranked = rank_candidates(scored)
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n = len(ranked)
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top_half_ids = {item.candidate.candidate_id for item in ranked[: n // 2]}
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for pid in POSITIVE_IDS:
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assert pid in top_half_ids, f"{pid} not in top half"
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def test_recall_at_5_equals_1(self):
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"""All 5 positives should appear in top-5 of 20 candidates."""
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scored, _ = score_candidates(MIXED_CANDIDATES)
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rc = recall_at_k(scored, POSITIVE_IDS, k=5)
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assert rc == 1.0, f"Expected recall@5=1.0, got {rc}"
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def test_enrichment_factor_at_5_exceeds_2(self):
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"""Pipeline should achieve at least 2× enrichment vs random at k=5."""
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scored, _ = score_candidates(MIXED_CANDIDATES)
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ef = enrichment_factor(scored, POSITIVE_IDS, k=5)
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assert ef >= 2.0, f"Enrichment factor {ef} below minimum of 2.0"
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def test_pipeline_verdict_outperforms_random(self):
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scored, _ = score_candidates(MIXED_CANDIDATES)
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summary = benchmark_summary(scored, POSITIVE_IDS, ks=[5])
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assert summary["verdict"] == "pipeline outperforms random"
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def test_negatives_score_lower_than_positives_on_average(self):
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scored, _ = score_candidates(MIXED_CANDIDATES)
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pos_scores = [
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s.scores["ensemble"]
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for s in scored
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if s.candidate.candidate_id in POSITIVE_IDS
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]
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neg_scores = [
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s.scores["ensemble"]
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for s in scored
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if s.candidate.candidate_id not in POSITIVE_IDS
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]
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assert pos_scores
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assert neg_scores
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assert sum(pos_scores) / len(pos_scores) > sum(neg_scores) / len(neg_scores)
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def test_bench_baseline_cli_shows_enrichment(self, tmp_path, capsys):
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out = str(tmp_path / "bench_report.json")
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ret = main([
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"bench", "baseline",
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"--candidates", MIXED_CANDIDATES,
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"--positives", ACTIVE_LABELS,
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"--k", "5",
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"--out", out,
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])
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assert ret == 0
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data = json.loads((tmp_path / "bench_report.json").read_text())
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k5_result = next(r for r in data["results"] if r["k"] == 5)
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assert k5_result["enrichment_factor"] >= 2.0
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assert data["verdict"] == "pipeline outperforms random"
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def test_benchmark_summary_disclaimer_present(self):
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scored, _ = score_candidates(MIXED_CANDIDATES)
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summary = benchmark_summary(scored, POSITIVE_IDS, ks=[5])
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assert "disclaimer" in summary
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assert len(summary["disclaimer"]) > 20
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def test_known_active_amps_outrank_all_repeat_sequences(self):
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"""Biologically implausible repeat sequences should rank below AMP-like ones."""
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scored, _ = score_candidates(MIXED_CANDIDATES)
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ranked = rank_candidates(scored)
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ranked_ids = [s.candidate.candidate_id for s in ranked]
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# All positives should appear before all-same-AA negatives
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for pos_id in POSITIVE_IDS:
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pos_rank = ranked_ids.index(pos_id)
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for neg_id in ["BM-NEG-001", "BM-NEG-002", "BM-NEG-004"]:
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neg_rank = ranked_ids.index(neg_id)
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assert pos_rank < neg_rank, (
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f"{pos_id} (rank {pos_rank}) should outrank {neg_id} (rank {neg_rank})"
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)
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class TestBenchmarkDataIntegrity:
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def test_benchmark_csvs_exist(self):
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from pathlib import Path
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assert Path(MIXED_CANDIDATES).exists()
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assert Path(ACTIVE_LABELS).exists()
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def test_all_positive_ids_in_mixed_candidates(self):
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mixed = load_candidates_csv(MIXED_CANDIDATES)
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mixed_ids = {c.candidate_id for c in mixed}
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for pid in POSITIVE_IDS:
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assert pid in mixed_ids, f"Positive {pid} missing from mixed candidates"
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def test_mixed_has_both_positives_and_negatives(self):
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mixed = load_candidates_csv(MIXED_CANDIDATES)
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ids = {c.candidate_id for c in mixed}
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positive_overlap = ids & POSITIVE_IDS
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negative_only = ids - POSITIVE_IDS
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assert len(positive_overlap) >= 3
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assert len(negative_only) >= 5
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def test_active_labels_are_subset_of_mixed(self):
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mixed = load_candidates_csv(MIXED_CANDIDATES)
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active = load_candidates_csv(ACTIVE_LABELS)
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mixed_ids = {c.candidate_id for c in mixed}
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for a in active:
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assert a.candidate_id in mixed_ids

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