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<!DOCTYPE html>
<html>
<head>
<meta charset="utf-8">
<title>Strobe-Enhanced Microscopy Stage</title>
<meta http-equiv="X-UA-Compatible" content="IE=edge">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel="shortcut icon" href="./assets/favicon.ico" />
<link href="./static/style.css" rel="stylesheet">
<link href="./assets/style.css" rel="stylesheet">
</head>
<body>
<header class="site-header">
<div class="wrapper header-wrapper" >
<div class=header-text>
<h1 class="site-title">Strobe-Enhanced Microscopy Stage</h1>
<p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado Labarca, and Tobias Wenzel</p>
<p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
</div>
</div>
</header>
<div class="page-content">
<div>
<nav class="sidebar">
<a href="#" class="menu-icon">
<svg viewBox="0 0 18 15">
<path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/>
<path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/>
<path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/>
</svg>
</a>
<ul class="nav-list">
<li>
<a class="navhome" href="./">
Strobe-Enhanced Microscopy Stage
</a>
</li>
<li class="active">
<a class="active" href="./2-level-station.html">
Build the 2-level microscope station
</a>
<ul class="sub-nav-list">
<li>
<a class="not-active" href="./2-level-station_BOM.html">
Bill of Materials
</a>
</li>
<li>
<a class="not-active" href="./printing.html">
Print the plastic parts
</a>
</li>
<li>
<a class="not-active" href="./laser-cutting.html">
Laser cut the acrylic parts
</a>
</li>
<li>
<a class="not-active" href="./assemble-focus-mechanism.html">
Assemble the focus mechanism
</a>
</li>
<li>
<a class="not-active" href="./basic-optics-module.html">
Assemble the basic optics module
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-top-plate.html">
Attach parts to the top plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-bottom-plate.html">
Attach parts to the middle plate
</a>
</li>
<li>
<a class="not-active" href="./mount-optics.html">
Mount the optics
</a>
</li>
<li>
<a class="not-active" href="./illumination.html">
Assemble the illumination
</a>
</li>
<li>
<a class="active" href="./wiring.html">
Complete the wiring
</a>
</li>
<li>
<a class="not-active" href="./finished.html">
Assembly complete
</a>
</li>
</ul>
</li>
<li class="not-active">
<a class="not-active" href="./software-installation.html">
Software Installation
</a>
</li>
<li class="not-active">
<a class="not-active" href="./usage.html">
Use your Station
</a>
</li>
</ul>
</nav>
</div>
<div class="wrapper">
<h1 id="complete-the-wiring">Complete the wiring</h1>
<p>These instructions assume you are using a Raspberry Pi v4, a Pi Hat, and the Strobe module.</p>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/tweezers.html">Tweezers</a> </li>
</ul>
<h3 id="consumable">Consumables</h3>
<ul>
<li>1 <a Class="bom" href="parts/consumables/gloves.html">Nitrile gloves</a> </li>
</ul>
<h3 id="electronic">Electronic Components</h3>
<ul>
<li>1 <a Class="bom" href="parts/elect/pi-hat.html">Pi Hat</a> - This is a custom open-source board documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li>
<li>1 <a Class="bom" href="parts/elect/rpi-v4.html">Raspberry Pi</a> </li>
<li>1 <a Class="bom" href="parts/elect/strobe-cable.html">Strobe Cable</a> - This is a custom connector documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li>
<li>1 <a Class="bom" href="parts/elect/strobe-module.html">Strobe Module</a> - This is a custom open-source board documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li>
</ul>
<h3 id="mechanic">Mechanical Components</h3>
</div>
<div class="caution-block">
<p><strong>Caution</strong> </p>
<p>Use <a href="parts/tools/tweezers.html">tweezers</a> and <a href="parts/consumables/gloves.html">gloves</a> to avoid directly touching the electronic components. This can avoid accidental damages.</p>
</div>
<h2 class="page-step" id="connect-the-camera">Step 1: Connect the camera</h2>
<ul>
<li>Insert the ribbon cable from the optics module into the camera port of the <a href="parts/elect/rpi-v4.html">Raspberry Pi</a>, ensuring the contacts are on the opposite side of the clasp. There are <a href="https://projects.raspberrypi.org/en/projects/getting-started-with-picamera/2" target="_blank" title="">detailed instructions on the Raspberry Pi website</a>.</li>
</ul>
<p><img alt="" src="images/RPi_RibbonCable.jpg" title="" /></p>
<h2 class="page-step" id="prepare-the-electronics">Step 2: Prepare the electronics</h2>
<ul>
<li>Place the <a href="parts/elect/strobe-module.html">strobe module</a> over the <a href="parts/elect/pi-hat.html">Pi hat</a>.</li>
<li>Mount the Pi hat on the Raspberry Pi GPIO headers. There is a space to place the ribbon cable.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/Pi-Hat_StrobeModule.jpg" alt="" />
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/RPi_Pi-Hat.jpg" alt="" />
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/RPi_Pi-Hat_1.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show0" src="images/Pi-Hat_StrobeModule.jpg" alt=""/></div>
<ul>
<li>Connect the 5-pin double row DuPont female connector of the <a href="parts/elect/strobe-cable.html">strobe cable</a> to the Pi hat.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/RPi_Pi-Hat_StrobeCable.jpg" alt="" />
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/RPi_Pi-Hat_StrobeCable_1.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show1" src="images/RPi_Pi-Hat_StrobeCable.jpg" alt=""/></div>
<hr />
<p><a href="illumination.html">Previous page</a> | <a href="finished.html">Next page</a></p>
</div>
</div>
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<span class="info">Documentation powered by GitBuilding</span>
</a>
<p class="author">©2023 Pierre Padilla-Huamantinco, Matías Hurtado Labarca, and Tobias Wenzel</p>
<p class="email">Contact: <a href="mailto:librehub@uc.cl">librehub@uc.cl</a></p>
<p class="license">Strobe-Enhanced Microscopy Stage is released under <a href="license.html">CERN-OHL-1.2</a>.</p>
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