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    <h1 class="site-title">Strobe-Enhanced Microscopy Stage</h1>
   
    <p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado Labarca, and Tobias Wenzel</p>
    <p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
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        Strobe-Enhanced Microscopy Stage
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        Build the 2-level microscope station
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                Bill of Materials
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                Print the plastic parts
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                Laser cut the acrylic parts
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                Assemble the focus mechanism
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                Assemble the basic optics module
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                Attach parts to the top plate
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                Attach parts to the middle plate
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                Mount the optics
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                Assemble the illumination
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                Complete the wiring
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                Assembly complete
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        Software Installation
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        Use your Station
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    <h1 id="print-the-plastic-parts">Print the plastic parts</h1>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/precision-wire-cutters.html">Precision wire cutter</a> </li>
<li>1 <a Class="bom" href="parts/tools/rep-rap.html">RepRap-style printer</a> </li>
<li>1 <a Class="bom" href="parts/tools/utility-knife.html">Utility knife</a>    - Not a scalpel!</li>
</ul>
<h3 id="material">Materials</h3>
<ul>
<li>50 g of <a Class="bom" href="parts/materials/black_pla_filament.html">Black PLA filament</a> </li>
<li>205 g of <a Class="bom" href="parts/materials/pla_filament.html">PLA filament</a>    - Of any colour you want</li>
</ul>
</div>
<h2 class="page-step" id="set-your-printer-settings">Step 1: Set your printer settings</h2>
<p>Almost all station parts can be printed out of <a href="parts/materials/pla_filament.html">PLA filament</a> on most <a href="parts/tools/rep-rap.html">RepRap-style printers</a>.</p>
<p>We recommend the following printer settings:</p>
<table>
<thead>
<tr>
<th>Setting</th>
<th>Value</th>
</tr>
</thead>
<tbody>
<tr>
<td>Material</td>
<td>PLA</td>
</tr>
<tr>
<td>Material Temperature</td>
<td>Recommended by the PLA brand</td>
</tr>
<tr>
<td>Layer height</td>
<td>0.2mm or less</td>
</tr>
<tr>
<td>Infill</td>
<td>Printer default</td>
</tr>
<tr>
<td>Brim</td>
<td>Recommended for all parts</td>
</tr>
<tr>
<td>Slice gap closing radius</td>
<td>0.001mm</td>
</tr>
</tbody>
</table>
<p>Test whether your printer can print the pieces for this station or other open-source designs. Download and print the <a href="models/leg_test.html">leg test</a> file. This will only use about <a href="parts/materials/pla_filament.html">5 grams of PLA</a>.</p>
<p>The result should look like this (this has been printed with a brim):</p>
<p><img alt="" src="images/just_leg_test.jpg" title="" /></p>
<p>As a general rule, strength is more important than surface finish, so very thin layers (less than 0.15mm or so) are unlikely to result in a station that performs any better, though it may approve the appearance.</p>
<h2 class="page-step" id="printing">Step 2: Printing</h2>
<p>Now you have tested your <a href="parts/tools/rep-rap.html">3D printer</a> and <a href="parts/materials/pla_filament.html">filament</a> you can print the following parts:</p>
<ul>
<li>Actuator assembly tools (<a name="output__nut-tool"></a>nut tool, <a name="output__band-tool"></a>band tool, and <a name="output__band-tool-cover"></a>band tool cover): <a href="models/actuator-assembly-tools.html" title="">actuator_assembly_tools.stl</a></li>
<li><a name="output__focusing-assembly"></a>Focusing assembly: <a href="models/focusing-assembly.html" title="">focusing-assembly.stl</a></li>
<li><a name="output__actuator-foot"></a>Actuator Foot: <a href="models/z_foot.html" title="">z_foot.stl</a></li>
<li><a name="output__thumbwheel"></a>Thumbwheel: <a href="models/thumbwheel.html" title="">thumbwheel.stl</a></li>
<li><a name="output__pi-camera-platform"></a>Pi camera platform: <a href="models/camera-platform.html" title="">camera-platform.stl</a></li>
<li><a name="output__lens-spacer"></a>Lens spacer: <a href="models/lens-spacer.html" title="">lens-spacer.stl</a> - <strong>This must be printed in <a href="parts/materials/black_pla_filament.html">black</a>!</strong></li>
<li><a name="output__illumination-base"></a>Illumination base: <a href="models/illumination-base.html" title="">illumination-base.stl</a> - <strong>This must be printed with supports</strong></li>
<li><a name="output__illumination-slider"></a>Illumination slider: <a href="models/illumination-slider.html" title="">illumination-slider.stl</a></li>
<li><a name="output__illumination-thumbscrew"></a>Illumination thumbscrew: <a href="models/illumination-thumbscrew.html" title="">illumination-thumbscrew.stl</a></li>
<li><a name="output__illumination-arm"></a>Illumination arm: <a href="models/led-arm.html" title="">led-arm.stl</a> - <strong>This must be printed with supports</strong></li>
<li><a name="output__hinge-pin"></a>Hinge pin: <a href="models/arm-pin.html" title="">arm-pin.stl</a></li>
<li><a name="output__led-cover"></a>LED cover: <a href="models/led-cover.html" title="">led-cover.stl</a></li>
<li><a name="output__standoff-s"></a>Standoff-S: <a href="models/standoff-S.html" title="">standoff-S.stl</a></li>
<li><a name="output__standoff-m"></a>Standoff-M: <a href="models/standoff-M.html" title="">standoff-M.stl</a></li>
<li><a name="output__foot-m"></a>Foot-M: <a href="models/foot-M.html" title="">foot-M.stl</a></li>
<li><a name="output__spacer-s"></a>Spacer-S: <a href="models/spacer-4mm.html" title="">spacer-4mm.stl</a></li>
<li><a name="output__spacer-m"></a>Spacer-M: <a href="models/spacer-11mm.html" title="">spacer-11mm.stl</a></li>
</ul>
<div class="info-block">
<p>For the printing do not use less than 15% infill.</p>
</div>
<p>You can <a href="models/STLs.rar" title="">download all the STLs as a single zipfile</a> (plates are not included).</p>
<h2 class="page-step" id="clean-up-of-printed-parts">Step 3: Clean-up of printed parts</h2>
<div class="warning-block">
<p><strong>Be careful when removing brim</strong></p>
<p>To avoid injury, first remove the bulk of the brim without a knife. Remove the remaining brim with a peeling action as described below.</p>
</div>
<p>Carefully remove the printing brim from all parts. To remove brim:</p>
<ol>
<li>Use <a href="parts/tools/precision-wire-cutters.html">precision wire cutters</a> to remove most of the brim from the part.</li>
<li>Clean up the remaining brim with a <a href="parts/tools/utility-knife.html">utility knife</a>:<ul>
<li>Hold the knife in your dominant hand with 4 fingers curled around the handle, leaving your thumb free.</li>
<li>Hold the part in your other hand, as far away from the surface, to be cut as possible.</li>
<li>Support the part with the thumb of your dominant hand.</li>
<li>Place the blade on the surface to be cut, and carefully close your dominant hand, moving the blade, under control, towards your thumb.</li>
</ul>
</li>
</ol>
<p><img alt="" src="images/BrimRemoval001.jpg" title="" /></p>
<hr />
<p><a href="2-level-station_BOM.html">Previous page</a> | <a href="laser-cutting.html">Next page</a></p>
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    <p class="author">©2023 Pierre Padilla-Huamantinco, Matías Hurtado Labarca, and Tobias Wenzel</p>
    <p class="email">Contact: <a href="mailto:librehub@uc.cl">librehub@uc.cl</a></p>
    <p class="license">Strobe-Enhanced Microscopy Stage is released under <a href="license.html">CERN-OHL-1.2</a>.</p>

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