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README.md

spatial-transcriptomics

Spatial transcriptomics analysis via ToolUniverse — gene expression mapping in tissue architecture for 10x Visium, MERFISH, seqFISH, Slide-seq, and imaging-based platforms.

What it does

Targets tooluniverse-spatial-transcriptomics agentic workflow. Performs spatial clustering, domain identification, cell-cell proximity analysis, spatially variable gene detection, tissue architecture mapping, and integration with single-cell data. Use for spatial expression patterns, tumor microenvironment spatial structure, or tissue organization studies.

⚠️ Current status

ToolUniverse wrapper — pip package doesn't bundle the agentic workflow. Script detects and reports this.

Setup

cd spatial-transcriptomics
python3 -m venv .venv && source .venv/bin/activate && pip install tooluniverse pyyaml -q

Environment variables

None for --list-workflows.

Usage

python3 scripts/run.py --help
python3 scripts/run.py --list-workflows
python3 scripts/run.py --query "Map spatial gene expression in Visium breast cancer"

Dependencies

tooluniverse, pyyaml

Tested with

  • --help:
  • Agno agent (Claude Haiku 4.5): ✅ Described spatial clustering, domain ID, cell-cell proximity, SVG detection, tissue architecture

Agno verdict

Perfect for understanding how cells organize in tissue and how their spatial relationships drive biological processes. Supports 10x Visium, MERFISH, seqFISH, Slide-seq, and imaging-based platforms.