Given that most markers are cell surface and not nuclear or cytoplasmic, why is default value of parameter basic_feature the mean?
As demonstrated by Automated Classification of Cellular Expression in Multiplexed Imaging Data with Nimbus, Nature Methods, this averaging leads to markers that are truly bimodal to appear as if they are unimodal and hence is misleadingly suggestive of the antibody being dodgy.
Given that most markers are cell surface and not nuclear or cytoplasmic, why is default value of parameter
basic_featurethe mean?As demonstrated by Automated Classification of Cellular Expression in Multiplexed Imaging Data with Nimbus, Nature Methods, this averaging leads to markers that are truly bimodal to appear as if they are unimodal and hence is misleadingly suggestive of the antibody being dodgy.